| Autor: |
Porto FL; Laboratory of Cell Biology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, Brazil.; Brazilian National Institute of Science and Technology on Neuroimmunomodulation (INCT-NIM), Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Lins MP; Brazilian National Institute of Science and Technology on Neuroimmunomodulation (INCT-NIM), Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.; Laboratory of Immunology, Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, Cuiabá, Brazil., Smaniotto S; Laboratory of Cell Biology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, Brazil.; Brazilian National Institute of Science and Technology on Neuroimmunomodulation (INCT-NIM), Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Reis MDDS; Laboratory of Cell Biology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, Brazil.; Brazilian National Institute of Science and Technology on Neuroimmunomodulation (INCT-NIM), Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. |
| Abstrakt: |
Thymic epithelial cells (TECs) dysfunction can lead to disorders in the adaptive immune response, resulting in immunodeficiency or autoimmune diseases. Therefore, the investigation of new drugs with immunomodulatory capacity can contribute to the development of strategies to improve thymic functions. In this context, this study aimed to investigate the in vitro effects of the pentacyclic triterpene friedelin (FD) on TEC biology. For this, murine 2BH4 cells were treated with 0.1 and 1 μM FD for 24 h. After treatment, fibronectin and laminin production was increased (16% and 37% respectively) by TECs, however it did not alter the expression of CXCL12 chemokine. The interaction between TEC and thymocytes was also evaluated, in which a greater adhesion (45%) and survival (228%) of thymocytes to treated-TECs was observed. MHC molecules were up-regulated by FD treatment plus thymocyte coculture. Based on these results it was possible to attest that FD has an important and promissory role in the physiology of murine TECs. |
