Engineering modular and tunable single-molecule sensors by decoupling sensing from signal output.

Autor: Grabenhorst L; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, Germany., Pfeiffer M; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, Germany., Schinkel T; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, Germany., Kümmerlin M; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, Germany., Brüggenthies GA; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, Germany., Maglic JB; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, Germany., Selbach F; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, Germany., Murr AT; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, Germany., Tinnefeld P; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, Germany. philip.tinnefeld@cup.uni-muenchen.de., Glembockyte V; Department of Chemistry and Center for NanoScience, Ludwig-Maximilians-Universität München, Munich, Germany. viktorija.glembockyte@mr.mpg.de.; Max Planck Institute for Medical Research, Heidelberg, Germany. viktorija.glembockyte@mr.mpg.de.
Jazyk: angličtina
Zdroj: Nature nanotechnology [Nat Nanotechnol] 2024 Nov 07. Date of Electronic Publication: 2024 Nov 07.
DOI: 10.1038/s41565-024-01804-0
Abstrakt: Biosensors play key roles in medical research and diagnostics. However, the development of biosensors for new biomolecular targets of interest often involves tedious optimization steps to ensure a high signal response at the analyte concentration of interest. Here we show a modular nanosensor platform that facilitates these steps by offering ways to decouple and independently tune the signal output as well as the response window. Our approach utilizes a dynamic DNA origami nanostructure to engineer a high optical signal response based on fluorescence resonance energy transfer. We demonstrate mechanisms to tune the sensor's response window, specificity and cooperativity as well as highlight the modularity of the proposed platform by extending it to different biomolecular targets including more complex sensing schemes. This versatile nanosensor platform offers a promising starting point for the rapid development of biosensors with tailored properties.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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