Altered genomic methylation promotes Staphylococcus aureus persistence in hospital environment.

Autor: Yamazaki Y; Department of Dermatology, Chiba University Graduate School of Medicine, 260-8670, Chiba, Japan.; Cutaneous Allergy and Host Defense, Immunology Frontier Research Center, Osaka University, 565-0871, Osaka, Japan.; Department of Dermatology, Osaka University Graduate School of Medicine, 565-0871, Osaka, Japan., Ito T; Department of Dermatology, Osaka University Graduate School of Medicine, 565-0871, Osaka, Japan., Nakagawa S; Department of Dermatology, Chiba University Graduate School of Medicine, 260-8670, Chiba, Japan.; Department of Pathology and Rogel Cancer Center, University of Michigan Medical School, MI, 48109, Ann Arbor, USA., Sugihira T; Department of Dermatology, Osaka University Graduate School of Medicine, 565-0871, Osaka, Japan., Kurita-Tachibana C; Cutaneous Allergy and Host Defense, Immunology Frontier Research Center, Osaka University, 565-0871, Osaka, Japan., Villaruz AE; Pathogen Molecular Genetics Section, Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20852, Bethesda, USA., Ishiguro K; Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 113-8656, Tokyo, Japan., Salcman B; Cutaneous Allergy and Host Defense, Immunology Frontier Research Center, Osaka University, 565-0871, Osaka, Japan., Li S; Cutaneous Allergy and Host Defense, Immunology Frontier Research Center, Osaka University, 565-0871, Osaka, Japan., Takada S; Department of Dermatology, Chiba University Graduate School of Medicine, 260-8670, Chiba, Japan., Inohara N; Department of Pathology and Rogel Cancer Center, University of Michigan Medical School, MI, 48109, Ann Arbor, USA., Kusuya Y; Medical Mycology Research Center, Chiba University, 260-8673, Chiba, Japan., Shibata A; Department of Infection Control Science, Graduate School of Pharmaceutical Sciences, Chiba University, 260-8675, Chiba, Japan., Tamai M; Department of Dermatology, Osaka University Graduate School of Medicine, 565-0871, Osaka, Japan., Aoyama R; Department of Dermatology, Osaka University Graduate School of Medicine, 565-0871, Osaka, Japan., Inoue K; Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, 567-0047, Osaka, Japan., Murata S; Division of Clinical Laboratory, Chiba University Hospital, 260-8677, Chiba, Japan., Matsushita K; Division of Clinical Laboratory, Chiba University Hospital, 260-8677, Chiba, Japan., Miyabe A; Division of Clinical Laboratory, Chiba University Hospital, 260-8677, Chiba, Japan., Taniguchi T; Division of Infection Control, Chiba University Hospital, 260-8677, Chiba, Japan., Igari H; Division of Infection Control, Chiba University Hospital, 260-8677, Chiba, Japan., Ishiwada N; Department of Infectious Diseases, Medical Mycology Research Center, Chiba University, 260-8673, Chiba, Japan., Taniguchi M; Department of Bio-Nanotechnology, The Institute of Scientific and Industrial Research, Osaka University, 565-0871, Osaka, Japan., Nakada TA; Department of Emergency and Critical Care Medicine, Chiba University Graduate School of Medicine, 260-8670, Chiba, Japan., Matsue H; Department of Dermatology, Chiba University Graduate School of Medicine, 260-8670, Chiba, Japan., Fujimoto M; Department of Dermatology, Osaka University Graduate School of Medicine, 565-0871, Osaka, Japan.; Cutaneous Immunology, Immunology Frontier Research Center, Osaka University, 565-0871, Osaka, Japan., Hishiki H; Department of Pediatrics, Chiba University Graduate School of Medicine, 260-8670, Chiba, Japan., Osone Y; Department of Pediatrics, Chiba University Graduate School of Medicine, 260-8670, Chiba, Japan., Hamada H; Department of Pediatrics, Chiba University Graduate School of Medicine, 260-8670, Chiba, Japan., Shimojo N; Department of Pediatrics, Chiba University Graduate School of Medicine, 260-8670, Chiba, Japan.; Center for Preventive Medical Sciences, Chiba University, 263-8522, Chiba, Japan., Suzuki T; Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 113-8656, Tokyo, Japan., Otto M; Pathogen Molecular Genetics Section, Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20852, Bethesda, USA., Núñez G; Department of Pathology and Rogel Cancer Center, University of Michigan Medical School, MI, 48109, Ann Arbor, USA., Takahashi H; Medical Mycology Research Center, Chiba University, 260-8673, Chiba, Japan.; Molecular Chirality Research Center, Chiba University, 263-8522, Chiba, Japan.; Plant Molecular Science Center, Chiba University, 260-8675, Chiba, Japan., Takaya A; Medical Mycology Research Center, Chiba University, 260-8673, Chiba, Japan.; Department of Infection Control Science, Graduate School of Pharmaceutical Sciences, Chiba University, 260-8675, Chiba, Japan.; Plant Molecular Science Center, Chiba University, 260-8675, Chiba, Japan., Nakamura Y; Cutaneous Allergy and Host Defense, Immunology Frontier Research Center, Osaka University, 565-0871, Osaka, Japan. ymatsuoka@derma.med.osaka-u.ac.jp.; Department of Dermatology, Osaka University Graduate School of Medicine, 565-0871, Osaka, Japan. ymatsuoka@derma.med.osaka-u.ac.jp.; Division of Microbiology and Immunology, Center for Infectious Disease Education and Research, Osaka University, 565-0871, Osaka, Japan. ymatsuoka@derma.med.osaka-u.ac.jp.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Nov 07; Vol. 15 (1), pp. 9619. Date of Electronic Publication: 2024 Nov 07.
DOI: 10.1038/s41467-024-54033-3
Abstrakt: Staphylococcus aureus can cause outbreaks and becomes multi-drug resistant through gene mutations and acquiring resistance genes. However, why S. aureus easily adapts to hospital environments, promoting resistance and recurrent infections, remains unknown. Here we show that a specific S. aureus lineage evolved from a clone that expresses the accessory gene regulator (Agr) system to subclones that reversibly suppressed Agr and caused an outbreak in the hospital setting. S. aureus with flexible Agr regulation shows increased ability to acquire antibiotic-resistant plasmids, escape host immunity, and colonize mice. Bacteria with flexible Agr regulation shows altered cytosine genomic methylation, including the decreased 5mC methylation in transcriptional regulator genes (pcrA and rpsD), compared to strains with normal Agr expression patterns. In this work, we discover how altered genomic methylation promotes flexible Agr regulation which is associated with persistent pathogen colonization in the hospital environment.
(© 2024. The Author(s).)
Databáze: MEDLINE
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