Metagenomic insight into the enrichment of antibiotic resistance genes in activated sludge upon exposure to nanoplastics.

Autor: Song J; Hunan Province Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, School of Resources Environment and Safety Engineering, University of South China, Hengyang, 421001, China. Electronic address: 15575468569@163.com., Huang Z; Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, School of Civil Engineering, University of South China, Hengyang, 421001, China., Gao Y; Hunan Province Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, School of Resources Environment and Safety Engineering, University of South China, Hengyang, 421001, China., Wang W; College of Environmental Science and Engineering, Nankai University, Tianjin, 300350, China., Guo G; Hubei Key Laboratory of Multi-media Pollution Cooperative Control in Yangtze Basin, School of Environmental Science and Engineering, Huazhong University of Science and Technology (HUST), Wuhan, 430074, China., Duan Y; Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, School of Civil Engineering, University of South China, Hengyang, 421001, China., Zhou S; Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, School of Civil Engineering, University of South China, Hengyang, 421001, China. Electronic address: zs402606665@126.com., Tang Z; Hunan Province Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, School of Resources Environment and Safety Engineering, University of South China, Hengyang, 421001, China. Electronic address: zptang01@126.com.
Jazyk: angličtina
Zdroj: Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2024 Dec 15; Vol. 363 (Pt 2), pp. 125260. Date of Electronic Publication: 2024 Nov 06.
DOI: 10.1016/j.envpol.2024.125260
Abstrakt: Activated sludge is an important reservoir for the co-occurring emerging contaminants including nanoplastics (NPs) and antibiotic resistance genes (ARGs). However, the impacts and potential mechanisms of NPs on the fate of ARGs in activated sludge are not fully understood. Herein, we used metagenomic approach to investigate the responses of ARGs, host bacteria, mobile genetic elements (MGEs), and functional genes to polystyrene (PS) NPs at environmentally relevant (0.5 mg/L) and high stress concentrations (50 mg/L) in activated sludge. The results showed that 0.5 and 50 mg/L PS NPs increased the relative abundance of ARGs in the activated sludge by 58.68% and 46.52%, respectively (p < 0.05). Host tracking analysis elucidated that the hosts of ARGs were significantly enriched by PS NPs (p < 0.05), with Proteobacteria being the predominant host bacteria. Additionally, the occurrence of new ARGs hosts and the enrichment of MGEs and functional genes (i.e., genes related to SOS response, cell membrane permeability, and secretion system, etc.) indicated that PS NPs promoted horizontal gene transfer (HGT) of ARGs. Finally, path modeling analysis revealed that the proliferation of ARGs caused by PS NPs was primarily attributed to the enhancement of HGT and the enrichment of host bacteria. Our findings contribute to a comprehensive understanding of the spread risk of ARGs in activated sludge under NPs pollution.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: