Enhanced pollen tube performance at high temperature contributes to thermotolerant fruit and seed production in tomato.

Autor:	Ouonkap SVY; Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, 60 Olive Street, Providence, RI 02912, USA., Palaniappan M; School of Plant Sciences, University of Arizona, 1140 E S Campus Drive, Forbes 303B, Tucson, AZ 85721, USA; Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625104, India., Pryze K; School of Plant Sciences, University of Arizona, 1140 E S Campus Drive, Forbes 303B, Tucson, AZ 85721, USA., Jong E; School of Plant Sciences, University of Arizona, 1140 E S Campus Drive, Forbes 303B, Tucson, AZ 85721, USA., Foteh Ali M; Department of Biology, Wake Forest University, 1834 Wake Forest Road, Winston-Salem, NC 27109, USA., Styler B; Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, 60 Olive Street, Providence, RI 02912, USA., Althiab Almasaud R; Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, 60 Olive Street, Providence, RI 02912, USA., Harkey AF; Department of Biology, Wake Forest University, 1834 Wake Forest Road, Winston-Salem, NC 27109, USA., Reid RW; Department of Bioinformatics and Genomics, The University of North Carolina at Charlotte, 9201 University City Blvd., Charlotte, NC 28223, USA., Loraine AE; Department of Bioinformatics and Genomics, The University of North Carolina at Charlotte, 9201 University City Blvd., Charlotte, NC 28223, USA., Smith SE; School of Natural Resources and the Environment, University of Arizona, 1064 E. Lowell Street, Tucson, AZ 85721, USA., Pease JB; Department of Biology, Wake Forest University, 1834 Wake Forest Road, Winston-Salem, NC 27109, USA; Department of Evolution, Ecology and Organismal Biology, The Ohio State University, 318 W. 12th Avenue, Columbus, OH 43210, USA., Muday GK; Department of Biology, Wake Forest University, 1834 Wake Forest Road, Winston-Salem, NC 27109, USA., Palanivelu R; School of Plant Sciences, University of Arizona, 1140 E S Campus Drive, Forbes 303B, Tucson, AZ 85721, USA. Electronic address: rpalaniv@arizona.edu., Johnson MA; Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, 60 Olive Street, Providence, RI 02912, USA. Electronic address: mark_johnson_1@brown.edu.
Jazyk:	angličtina
Zdroj:	Current biology : CB [Curr Biol] 2024 Nov 18; Vol. 34 (22), pp. 5319-5333.e5. Date of Electronic Publication: 2024 Nov 06.
DOI:	10.1016/j.cub.2024.10.025
Abstrakt:	Rising temperature extremes during critical reproductive periods threaten the yield of major grain and fruit crops. Flowering plant reproduction depends on the ability of pollen grains to generate a pollen tube, which elongates through the pistil to deliver sperm cells to female gametes for double fertilization. We used tomato as a model fruit crop to determine how high temperature affects the pollen tube growth phase, taking advantage of cultivars noted for fruit production in exceptionally hot growing seasons. We found that exposure to high temperature solely during the pollen tube growth phase limits fruit biomass and seed set more significantly in thermosensitive cultivars than in thermotolerant cultivars. Importantly, we found that pollen tubes from the thermotolerant Tamaulipas cultivar have enhanced growth in vivo and in vitro under high temperature. Analysis of the pollen tube transcriptome's response to high temperature allowed us to define two response modes (enhanced induction of stress responses and higher basal levels of growth pathways repressed by heat stress) associated with reproductive thermotolerance. Importantly, we define key components of the pollen tube stress response, identifying enhanced reactive oxygen species (ROS) homeostasis and pollen tube callose synthesis and deposition as important components of reproductive thermotolerance in Tamaulipas. Our work identifies the pollen tube growth phase as a viable target to enhance reproductive thermotolerance and delineates key pathways that are altered in crop varieties capable of fruiting under high-temperature conditions. Competing Interests: Declaration of interests R.P. is the editor-in-chief of Plant Reproduction. (Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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