Seasonal Variation in ATP-Induced Retinal Damage in the Cone-Dominant 13-Lined Ground Squirrel.

Autor:	Bowie OR; School of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA., Follett HM; Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, Milwaukee, WI, USA., Yu CT; Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, Milwaukee, WI, USA., Guillaume C; School of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA., Summerfelt PM; Ophthalmology & Visual Sciences, Medical College of Wisconsin, Milwaukee, WI, USA., Manfredonia N; School of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA., Grieshop J; Ophthalmology & Visual Sciences, Medical College of Wisconsin, Milwaukee, WI, USA.; Joint Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin, Milwaukee, WI, USA., Merriman DK; Department of Biology, University of Wisconsin Oshkosh, Oshkosh, WI, USA., Tarima S; Division of Biostatistics, Institute for Health and Equity, Medical College of Wisconsin, Milwaukee, WI, USA., Carroll J; Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, Milwaukee, WI, USA.; Ophthalmology & Visual Sciences, Medical College of Wisconsin, Milwaukee, WI, USA.; Joint Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin, Milwaukee, WI, USA.
Jazyk:	angličtina
Zdroj:	Translational vision science & technology [Transl Vis Sci Technol] 2024 Nov 04; Vol. 13 (11), pp. 5.
DOI:	10.1167/tvst.13.11.5
Abstrakt:	Purpose: To examine whether time of year (relative to hibernation emergence) influences the retinal degenerative effects of intravitreal injection of adenosine triphosphate (ATP) in the 13-lined ground squirrel (13-LGS). Methods: Eighteen (9 male, 9 female) 13-LGS in three experimental cohorts (early season, mid-season, late season) (n = 6 each) underwent baseline imaging using scanning light ophthalmoscopy (SLO) and optical coherence tomography (OCT). Animals then received a 10-µL intravitreal injection of 0.723 M ATP, followed by OCT and SLO imaging at 3, 10, and 21 days. Adaptive optics SLO (AOSLO) was performed in animals without retinal damage after the 21-day follow-up. Retinal thickness, choroidal thickness, and cone density measures were compared to values from wild-type controls (n = 12). Results: Five animals (four early season, one late season) showed retinal damage post-ATP injection (Fisher's exact test, P = 0.065). Animals with retinal damage displayed areas of disrupted retinal lamination on OCT. Any changes in OCT thickness were generally present on initial follow-up and resolved at later time points. Follow-up imaging with AOSLO on animals without retinal damage showed no significant differences in the cone mosaic topography from control eyes. Axial length was increased in mid-/late-season cohorts relative to early season (P = 0.0025 and P = 0.0007). Conclusions: In this pilot study, the 13-LGS appears more susceptible to ATP-induced retinal damage during the early season. Future studies adjusting dose based on ocular biometry may help elucidate the impact of time of year on chemical response. Translational Relevance: Consideration of ocular biometry in this and other animal models is merited when using intravitreal methods of chemical administration.
Databáze:	MEDLINE
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