Host-derived Lactobacillus plantarum alleviates hyperuricemia by improving gut microbial community and hydrolase-mediated degradation of purine nucleosides.

Autor:	Fu Y; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Luo XD; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Li JZ; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Mo QY; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Wang X; State Key Laboratory of Microbial Technology, Shandong University, Shandong, China., Zhao Y; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Zhang YM; State Key Laboratory of Microbial Technology, Shandong University, Shandong, China., Luo HT; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Xia DY; School of Marine Sciences, Sun Yat-sen University, and Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China., Ma WQ; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Chen JY; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Wang LH; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Deng QY; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Ben L; International Livestock Research Institute, Nairobi, Kenya., Kashif Saleemi M; Department of Pathology, University of Agriculture Faisalabad, Faisalabad, Pakistan., Jiang XZ; Microbiome Research Center, Moon (Guangzhou) Biotech Co. Ltd, Guangdong, China., Chen J; Microbiome Research Center, Moon (Guangzhou) Biotech Co. Ltd, Guangdong, China., Miao K; CancerCenter, Faculty of Health Sciences, University of Macau, Macau, China., Lin ZP; Shantou Baisha Research Institute of Origin Species of Poultry and Stock, Shantou, China., Zhang P; Chimelong Safari Park, Chimelong Group Co, Guangzhou, China., Ye H; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Cao QY; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Zhu YW; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Yang L; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China., Tu Q; Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.; Shenzhen Key Laboratory of Genome Manipulation and Biosynthesis, CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China., Wang W; State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2024 Nov 07; Vol. 13. Date of Electronic Publication: 2024 Nov 07.
DOI:	10.7554/eLife.100068
Abstrakt:	The gut microbiota is implicated in the pathogenesis of hyperuricemia (HUA) and gout. However, it remains unclear whether probiotics residing in the host gut, such as Lactobacillus , can prevent HUA development. Herein, we isolated Lactobacillus plantarum SQ001 from the cecum of HUA geese and conducted in vitro assays on uric acid (UA) and nucleoside co-culture. Metabolomics and genome-wide analyses, revealed that this strain may promote nucleoside uptake and hydrolysis through its nucleoside hydrolase gene. The functional role of iunH gene was confirmed via heterologous expression and gene knockout studies. Oral administration of L. plantarum SQ001 resulted in increased abundance of Lactobacillus species and reduced serum UA levels. Furthermore, it downregulated hepatic xanthine oxidase, a key enzyme involved in UA synthesis, as well as renal reabsorption protein GLUT9, while enhancing the expression of renal excretion protein ABCG2. Our findings suggest that L. plantarum has potential to ameliorate gut microbial dysbiosis with HUA, thereby offering insights into its potential application as a probiotic therapy for individuals with HUA or gout. Competing Interests: YF, XL, JL, QM, XW, YZ, YZ, HL, DX, WM, JC, LW, QD, LB, MK, KM, ZL, HY, QC, YZ, LY, QT, WW No competing interests declared, XJ, JC Affiliated with Biotech Co. Ltd; the author has no financial interests to declare, PZ Affiliated with Chimelong Group Co; the author has no financial interests to declare (© 2024, Fu et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu