[Expression of circRNA_051778 in Lung Adenocarcinoma-Associated Malignant and Tuberculous Pleural Effusions and Its Clinical Significance].
| Autor: | Ye Z; （ 610041） West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China., Nong X; （ 610041） West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China., Wang Y; （ 610041） West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China., Che G; （ 610041） West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China., Zhou B; （ 610041） West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China., Huang J; （ 610041） West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China., Zhang L; （ 610041） West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China.; （ 330006） Department of Pathology, Jiangxi Chest Hospital, Nanchang 330006, China. |
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| Jazyk: | čínština |
| Zdroj: | Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2024 Sep 20; Vol. 55 (5), pp. 1254-1263. |
| DOI: | 10.12182/20240960302 |
| Abstrakt: | Objective: To investigate the expression and clinical significance of circular RNA (circRNA) 051778 in lung adenocarcinoma-malignant pleural effusion (LA-MPE) and tuberculous pleural effusion (TPE). Methods: This is a cross-sectional study. A total of 212 patients were recruited from the Jiangxi Chest Hospital between October 2018 and September 2019, and their pleural effusion samples and/or plasma samples were collected. The exosomal circRNA profile was sketched by circRNA microarray. Differentially expressed circRNAs (DECs) were verified by droplet digital PCR. In addition, a putative circRNA-miRNA-mRNA network was constructed, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to predict the functions of the DECs. The diagnostic value of circRNA_051778 was evaluated by binary logistic regression and receiver operating characteristic curve. Results: The expression level of circRNA_051778 in the LA-MPE samples was (3.92±0.48) copies/100 ng cDNA, while that in the TPE samples was (21.53±2.22) copies/100 ng cDNA. Compared to that in the TPE samples, circRNA_051778 was significantly downregulated in the LA-MPE samples ( P <0.001). The potential targets of circRNA_051778 were enriched in positive regulation of GTPase activity, cytoplasm, protein binding, and cancer-related pathways. The area under the curve (AUC) for the combined assessment of circRNA_051778 with liquid-based thin-layer cytology (TCT), erythrocyte sedimentation rate (ESR), and tuberculosis antibody (TBA) was 0.98 (95% confidence interval: 0.97-1.00), with the sensitivity being 88.0% and the specificity being 100.0%. Conclusion: Exosomal circRNA_051778 is downregulated in LA-MPE. According to the findings from the GO and KEGG analyses, exosomal circRNA_051778 may play a role in cancer development and has the potential to serve as a marker for differential diagnostic of LA-MPE and TPE when it is used in combination with TCT, ESR, and TBA. Competing Interests: 利益冲突 本文作者张林是本刊主编。该文在编辑评审过程中所有流程严格按照期刊政策进行，且未经其本人经手处理。除此之外，所有作者声明不存在利益冲突。 (© 2024《四川大学学报（医学版）》编辑部 版权所有Copyright ©2024 Editorial Office of Journal of Sichuan University (Medical Sciences).) |
| Databáze: | MEDLINE |
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