Arabidopsis GDH1 and GDH2 genes double knock-out results in a stay-green phenotype during dark-induced senescence.

Autor: Garnik EY; Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontova Str. 132, Irkutsk, Russia 664033., Vilyanen DV; Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontova Str. 132, Irkutsk, Russia 664033.; Present Address: Institute of Basic Biological Problems RAS, Federal Research Center, Pushchino Scientific Center for Biological Research RAS, Prospekt Nauki 3, Pushchino, Moscow Region, Russia 142290., Vlasova AA; Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontova Str. 132, Irkutsk, Russia 664033.; Present Address: Irkutsk State University, Karl Marx Str. 1, Irkutsk, Russia 664003., Tarasenko VI; Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontova Str. 132, Irkutsk, Russia 664033., Konstantinov YM; Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontova Str. 132, Irkutsk, Russia 664033.; Present Address: Irkutsk State University, Karl Marx Str. 1, Irkutsk, Russia 664003.
Jazyk: angličtina
Zdroj: Physiology and molecular biology of plants : an international journal of functional plant biology [Physiol Mol Biol Plants] 2024 Oct; Vol. 30 (10), pp. 1631-1642. Date of Electronic Publication: 2024 Oct 17.
DOI: 10.1007/s12298-024-01517-7
Abstrakt: Yellowing is the first visually observable sign of plant leaf senescence. We found that Arabidopsis double knockout mutant gdh1gdh2 for genes of NAD(H)-dependent glutamate dehydrogenase retains green color of the leaves (stay-green phenotype) during a dark-induced senescence, in contrast to wild-type plants, whose leaves turn yellow. When the gdh1gdh2 plants are exposed to the dark more than four days, they demonstrate slower chlorophyll degradation than in the wild-type plants under the same conditions, as well as dysregulation of chlorophyll breakdown genes encoding chlorophyll b reductase, Mg-dechelatase, pheophytinase and pheophorbide a oxygenase. The slowed degradation of chlorophyll b in gdh1gdh2 plants significantly alters the chlorophyll a / b ratio. Ion leakage in the mutant plants increases significantly from four to eight days in the darkness, correlating with their premature death during this period. The discovered facts suggest a functional connection between activity of NAD(H)-dependent glutamate dehydrogenase and dark-induced senescence progress in Arabidopsis.
Competing Interests: Conflict of interestThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© Prof. H.S. Srivastava Foundation for Science and Society 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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