Quantitative Assessment of Collagen Architecture to Determine Role of Tumor Stroma During Vestibular Schwannoma Progression.

Autor: Fisher M; Department of Otolaryngology-Head and Neck Surgery, Division of Otology, Neurotology and Cranial Base Surgery, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA., Duhon BH; Department of Otolaryngology-Head and Neck Surgery, Division of Otology, Neurotology and Cranial Base Surgery, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA., Nguyen HTN; Department of Otolaryngology-Head and Neck Surgery, Division of Otology, Neurotology and Cranial Base Surgery, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA., Tonniges JR; Campus Microscopy and Imaging Facility, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, USA., Wu KC; Department of Neurosurgery, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA., Ren Y; Department of Otolaryngology-Head and Neck Surgery, Division of Otology, Neurotology and Cranial Base Surgery, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
Jazyk: angličtina
Zdroj: Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery [Otolaryngol Head Neck Surg] 2024 Nov 07. Date of Electronic Publication: 2024 Nov 07.
DOI: 10.1002/ohn.1018
Abstrakt: Objective: The primary objective was to characterize the abundance and architecture of collagen in the extracellular matrix in vestibular schwannoma (VS). The secondary objective was to investigate the association between collagen architecture and tumor size.
Study Design: Retrospective cohort study.
Setting: Academic referral center.
Methods: Tumor samples were obtained from patients with sporadic VS undergoing microsurgical resection. Histological analyses were performed including picrosirius red (PSR) staining under polarized light. Collagen architecture was quantified using an automated fiber detection software. Second Harmonic Generation (SHG) microscopy and immunofluorescence (IF) were utilized to characterize collagen architecture.
Results: Eleven tumor specimens were included (mean tumor diameter = 2.80 cm, range 1.5-4.0 cm), and were divided into large (mean diameter = 3.5 ± 0.4 cm) and small (mean tumor diameter = 2.0 ± 0.4 cm) cohorts based on size. The large VS cohort showed significantly higher collagen density (27.65% vs 12.73%, P = .0043), with more thick fibers (mature Type I, 24.54% vs 12.97%, P = .0022) and thin fibers (immature Type I or mature Type III, 23.55% vs 12.27%, P = .026). Tumor volume correlated with greater degree of collagen fiber disorganization (P = .0413, r 2  = 0.298). Specifically, collagen type I intensity was significantly higher in large VS compared to small tumors (P < .001) and peripheral nerve (P = .028).
Conclusion: Larger VS exhibit increased collagen abundance in the tumor stroma, and a more disorganized collagen architecture compared to smaller VS and normal peripheral nerve tissue. This finding indicates that collagen organization may play a significant role in extracellular matrix remodeling and the progression of VS.
(© 2024 The Author(s). Otolaryngology–Head and Neck Surgery published by Wiley Periodicals LLC on behalf of American Academy of Otolaryngology–Head and Neck Surgery Foundation.)
Databáze: MEDLINE
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