Long-term safety of photobiomodulation exposure to beta cell line and rat islets in vitro and in vivo.

Autor: Perrier Q; University Grenoble Alpes, INSERM U1055, Pharmacy department, Grenoble Alpes University Hospital, LBFA, Grenoble, France. qperrier@chu-grenoble.fr., Cottet-Rousselle C; University Grenoble Alpes, INSERM U1055, LBFA, Grenoble, France., Lamarche F; University Grenoble Alpes, INSERM U1055, LBFA, Grenoble, France., Tubbs E; University Grenoble Alpes, CEA, INSERM, IRIG-BGE UA13, BIOMICS, Grenoble, France., Tellier C; University Grenoble Alpes, INSERM U1055, LBFA, Grenoble, France., Veyrat J; University Grenoble Alpes, INSERM U1055, LBFA, Grenoble, France., Vial G; University Grenoble Alpes, INSERM U1300, Grenoble Alpes University Hospital, HP2, Grenoble, France., Bleuet P; University Grenoble Alpes, CEA, LETI, Clinatec, Grenoble, France., Durand A; University Grenoble Alpes, Division for Biology and Healthcare Technologies, Microfluidic Systems and Bioengineering Lab, CEA, Leti, Grenoble, France., Pitaval A; University Grenoble Alpes, CEA, INSERM, IRIG-BGE UA13, BIOMICS, Grenoble, France., Cosnier ML; University Grenoble Alpes, Division for Biology and Healthcare Technologies, Microfluidic Systems and Bioengineering Lab, CEA, Leti, Grenoble, France., Moro C; University Grenoble Alpes, CEA, LETI, Clinatec, Grenoble, France., Lablanche S; University Grenoble Alpes, INSERM U1055, Diabetology and endocrinology department, Grenoble Alpes University Hospital, LBFA, Grenoble, France.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 06; Vol. 14 (1), pp. 26874. Date of Electronic Publication: 2024 Nov 06.
DOI: 10.1038/s41598-024-77660-8
Abstrakt: This study evaluates the safety and potential benefits of PBM on pancreatic beta cells and islets. PBM was applied to insulin-secreting cell lines (MIN6) and rat pancreatic islets using a 670 nm light source, continuous output, with a power density of 2.8 mW/cm², from 5 s to several 24 h. Measure of cell viability, insulin secretion, mitochondrial function, ATP content, and cellular respiration were assessed. Additionally, a diabetic rat model is used for islet transplantation (pre-conditioning with PBM or not) experiments. Short and long-term PBM exposure did not affect beta cell islets viability, insulin secretion nor ATP content. While short-term PBM (2 h) increases superoxide ion content, this was not observed for long exposure (24 h). Mitochondrial respirations were slightly decreased after PBM. In the islet transplantation model, both pre-illuminated and non-illuminated islets improved metabolic control in diabetic rats with a safety profile regarding the post-transplantation period. In summary, for the first time, long-term PBM exhibited safety in terms of cell viability, insulin secretion, energetic profiles in vitro, and post-transplantation period in vivo. Further investigation is warranted to explore PBM's protective effects under conditions of stress, aiding in the development of innovative approaches for cellular therapy.
(© 2024. The Author(s).)
Databáze: MEDLINE
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