Genome-based classification of Halobellus rubicundus sp. nov., a novel extremely halophilic archaeon isolated from a Korean solar saltern.

Autor: Hwang CY; Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea., Cho ES; Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea.; Biotechnology Institute, University of Minnesota, St. Paul, MN, 55108, USA., Cha IT; Climate Change and Environmental Biology Research Division, National Institute of Biological Resources, Incheon, 22689, Republic of Korea., Lee KE; Microorganism Resources Division, National Institute of Biological Resources, Incheon, 22689, Republic of Korea., Lee EY; Microorganism Resources Division, National Institute of Biological Resources, Incheon, 22689, Republic of Korea., Seo MJ; Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea. mjseo@inu.ac.kr.; Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea. mjseo@inu.ac.kr.; Research Center for Bio Materials & Process Development, Incheon National University, Incheon, 22012, Republic of Korea. mjseo@inu.ac.kr.
Jazyk: angličtina
Zdroj: Extremophiles : life under extreme conditions [Extremophiles] 2024 Nov 06; Vol. 28 (3), pp. 49. Date of Electronic Publication: 2024 Nov 06.
DOI: 10.1007/s00792-024-01367-x
Abstrakt: A novel extremely halophilic archaeon designated, MBLA0158 T , was isolated from a solar saltern in Sorae, Republic of Korea. The colonies are red-pigmented, Gram-stain-negative, pleomorphic, non-motile, and lysed in distilled water. The strain grows at 25-45 °C (optimum, 37 °C), in 15-30% (w/v) NaCl (optimum, 20%) and 0.1-1.0 M Mg 2+ (optimum, 0.2-0.3 M) at pH 6.0-10.0 (optimum, 7.0-8.0). Comparative analysis based on the 16S rRNA gene sequence revealed that this strain is most closely related to the Halobellus inordinatus YC20 T with a sequence identity of 96.0%. Strain MBLA0158 T contained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester as major polar lipids. The genome size is 3.29 Mb and the DNA G + C content is 66.9 mol%. Phylogenomic analysis confirmed that strain MBLA0158 T is distinct from previously reported type strains of the genus Halobellus. Pan-genome analysis showed that strain MBLA0158 T contains 419 genes that are not present in other type strains of the genus Halobellus. Based on overall analyses, strain MBLA0158 T is considered to represent a new species of the genus Halobellus, for which the name Halobellus rubicundus sp. nov. is proposed. The type strain is MBLA0158 T (= KCTC 4318 T  = JCM 36642 T ).
(© 2024. The Author(s), under exclusive licence to Springer Nature Japan KK.)
Databáze: MEDLINE
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