Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment.

Autor: Kroupová Z; Institute of Food Science and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic. Zuzana.Kroupova@vut.cz., Slaninová E; Institute of Food Science and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic., Mrázová K; Institute of Scientific Instruments of the Czech Academy of SciencesV.V.I., Kralovopolska 147, 612 64, Brno, Czech Republic., Krzyžánek V; Institute of Scientific Instruments of the Czech Academy of SciencesV.V.I., Kralovopolska 147, 612 64, Brno, Czech Republic., Hrubanová K; Institute of Scientific Instruments of the Czech Academy of SciencesV.V.I., Kralovopolska 147, 612 64, Brno, Czech Republic., Fritz I; Institute of Environmental Biotechnology, Department of Agrobiotechnology, IFA-Tulln, University of Natural Resources and Life Sciences, Konrad-Lorenz-Strasse 20, 3430, Tulln an Der Donau, Austria., Obruča S; Institute of Food Science and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic.
Jazyk: angličtina
Zdroj: Folia microbiologica [Folia Microbiol (Praha)] 2024 Nov 06. Date of Electronic Publication: 2024 Nov 06.
DOI: 10.1007/s12223-024-01212-w
Abstrakt: Cyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With their ability to fix CO 2 , cyanobacteria are interesting for white biotechnology as cell factories to produce various high-value metabolites such as polyhydroxyalkanoates, pigments, or proteins. White biotechnology is the industrial production and processing of chemicals, materials, and energy using microorganisms. It is known that exposing cyanobacteria to low levels of stressors can induce the production of secondary metabolites. Understanding of this phenomenon, known as hormesis, can involve the strategic application of controlled stressors to enhance the production of specific metabolites. Consequently, precise measurement of cyanobacterial viability becomes crucial for process control. However, there is no established reliable and quick viability assay protocol for cyanobacteria since the task is challenging due to strong interferences of autofluorescence signals of intercellular pigments and fluorescent viability probes when flow cytometry is used. We performed the screening of selected fluorescent viability probes used frequently in bacteria viability assays. The results of our investigation demonstrated the efficacy and reliability of three widely utilised types of viability probes for the assessment of the viability of Synechocystis strains. The developed technique can be possibly utilised for the evaluation of the importance of polyhydroxyalkanoates for cyanobacterial cultures with respect to selected stressor-repeated freezing and thawing. The results indicated that the presence of polyhydroxyalkanoate granules in cyanobacterial cells could hypothetically contribute to the survival of repeated freezing and thawing.
(© 2024. The Author(s).)
Databáze: MEDLINE
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