Optimization of microwave-assisted extraction using response surface methodology and HPLC-DAD phenolic compounds quantification from Hylocereus undatus peel and pulp cultivated in Tunisia.

Autor: Ayouni W; Organic Chemistry Laboratory LR17ES08, Sciences of Sfax Faculty, Chemistry Department, University of Sfax, Tunisia., Riguene H; Organic Chemistry Laboratory LR17ES08, Sciences of Sfax Faculty, Chemistry Department, University of Sfax, Tunisia., Yahyaoui A; Organic Chemistry Laboratory LR17ES08, Sciences of Sfax Faculty, Chemistry Department, University of Sfax, Tunisia., Dhiabi M; Applied Chemistry Laboratory: Heterocycles, Compounds, and Polymers, Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia., Dali S; Organic Chemistry Laboratory LR17ES08, Sciences of Sfax Faculty, Chemistry Department, University of Sfax, Tunisia.; Higher Institute of Applied and Technological Sciences of Gabes, University of Gabes, Gabes, Tunisia., Ammar H; Applied Chemistry Laboratory: Heterocycles, Compounds, and Polymers, Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia., Choura S; Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Chamkha M; Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Ben Salem R; Organic Chemistry Laboratory LR17ES08, Sciences of Sfax Faculty, Chemistry Department, University of Sfax, Tunisia., Ghayth R; Organic Chemistry Laboratory LR17ES08, Sciences of Sfax Faculty, Chemistry Department, University of Sfax, Tunisia.; Faculty of Sciences and Technology of Sidi Bouzid, Department of Physics & Chemistry, University of Kairouan, Tunisia.
Jazyk: angličtina
Zdroj: Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2024 Nov 06, pp. 1-17. Date of Electronic Publication: 2024 Nov 06.
DOI: 10.1080/10826068.2024.2423636
Abstrakt: The present study aimed to optimize the microwave-assisted extraction process for both the peel and pulp of Hylocereus undatus (white dragon fruit) cultivated in Tunisia, using response surface methodology. Total phenolic content, total flavonoid content, FRAP (ferric reducing antioxidant power), and DPPH (1,1-diphenyl-2-picrylhydrazyl) antioxidant activities were optimized. A central composite design (CCD) was applied, considering three key variables: extraction time, extraction temperature, and liquid-to-solid ratio. The optimized extraction parameters for Hylocereus undatus peel and pulp were determined respectively (9.57 min, 42.20 °C and 27.79 mL/g) and (10.08 min, 40.84 °C and 31.52 mL/g). The main phenolic compounds identified in Hylocereus undatus peel and pulp extracts using HPLC-DAD were chlorogenic and caffeic acids and rutin, quercetin, luteolin-7- O -glucoside as flavonoids. Therefore, this research has revealed the potential of a sustainable and eco-friendly process hold promise a directional option and encouraging a circular economy approach for industrial production of antioxidant-rich Hylocereus undatus extracts.
Databáze: MEDLINE