Apoptosis, inflammatory and innate immune responses induced by infection with a novel goose astrovirus in goose embryonic kidney cells.

Autor:	Hou Z; College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.; Waterfowl Healthy Breeding Engineering Research Center of Guangdong, Zhongkai University of Agriculture and Engineering, Guangzhou, China., Jin S; College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.; Waterfowl Healthy Breeding Engineering Research Center of Guangdong, Zhongkai University of Agriculture and Engineering, Guangzhou, China., Liang Y; College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.; Waterfowl Healthy Breeding Engineering Research Center of Guangdong, Zhongkai University of Agriculture and Engineering, Guangzhou, China., Wang H; College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.; Waterfowl Healthy Breeding Engineering Research Center of Guangdong, Zhongkai University of Agriculture and Engineering, Guangzhou, China., Jiang D; College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.; Waterfowl Healthy Breeding Engineering Research Center of Guangdong, Zhongkai University of Agriculture and Engineering, Guangzhou, China., Cao N; College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.; Waterfowl Healthy Breeding Engineering Research Center of Guangdong, Zhongkai University of Agriculture and Engineering, Guangzhou, China., Sun M; Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China., Tian Y; College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.; Waterfowl Healthy Breeding Engineering Research Center of Guangdong, Zhongkai University of Agriculture and Engineering, Guangzhou, China., Liu W; College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.; Waterfowl Healthy Breeding Engineering Research Center of Guangdong, Zhongkai University of Agriculture and Engineering, Guangzhou, China., Xu D; College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.; Waterfowl Healthy Breeding Engineering Research Center of Guangdong, Zhongkai University of Agriculture and Engineering, Guangzhou, China., Fu X; College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.; Waterfowl Healthy Breeding Engineering Research Center of Guangdong, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 Oct 22; Vol. 14, pp. 1452158. Date of Electronic Publication: 2024 Oct 22 (Print Publication: 2024).
DOI:	10.3389/fcimb.2024.1452158
Abstrakt:	Introduction: Since 2016, a highly lethal visceral gout induced by infection with the novel goose astrovirus (GoAstV) resulted in an ongoing outbreak in goslings in China, with a mortality rate ranging from 10% to 50%, and causing considerable economic losses in the goose industry. However, the pathogenesis of GoAstV and the molecular mechanism by which kidney lesions are induced by GoAstV infection are unclear. Methods: In the present study, a GEK cell infection model for GoAstV was established, and the apoptosis, inflammatory and innate immune responses induced by GoAstV were investigated in GEK cells. Results: The results shown that the expression of proapoptotic proteins, including Bax, caspase-3, caspase-9 and cytochrome c , increased in the infection group; however, the expression of the antiapoptotic protein Bcl-2 decreased, indicating that apoptosis was induced by GoAstV infection in GEK cells. Besides, the activation of the RIG-I/MDA5 pathway and the downstream upregulation of proinflammatory cytokines, including the adapter proteins MAVS, IRF7 and NF-κB and the proinflammatory cytokines IL-6, IL-8 and TNF-α, were detected in GEK cells infected with GoAstV. In addition, GoAstV infection induces the activation of the NLPR3 pathway and further stimulates the increased production of IL-1β. In summary, the present study revealed that GoAstV infection could induce apoptosis and the activation of the RIG-I/MDA5 and NLRP3 pathways in GEK cells, as well as the massive release of proinflammatory cytokines. Discussion: These results are helpful for elucidating the molecular mechanism of pathological lesions in the kidney in gout goslings infected with GoAstV and the interaction between GoAstV and the innate immune system of the host. Competing Interests: The authors declare the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Hou, Jin, Liang, Wang, Jiang, Cao, Sun, Tian, Liu, Xu and Fu.)
Databáze:	MEDLINE
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