Decreased DNA density is a better indicator of a nuclear bleb than lamin B loss.

Autor:	Bunner S; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Prince K; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA.; Molecular and Cellular Biology, University of Massachusetts Amherst, Amherst, MA 01003, USA., Pujadas Liwag EM; Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.; IBIS Interdisciplinary Biological Sciences Graduate Program, Northwestern University, Evanston, IL 60208, USA., Eskndir N; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Srikrishna K; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., McCarthy AA; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Kuklinski A; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Jackson O; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Pellegrino P; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Jagtap S; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Eweka I; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Lawlor C; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Eastin E; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Yas G; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Aiello J; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., LaPointe N; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., von Blucher IS; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Hardy J; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Chen J; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Figueroa S; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Backman V; Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA., Janssen A; School of Biological Sciences, University of Cambridge, Cambridge, UK., Packard M; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Dorfman K; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA., Almassalha L; Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA., Seifu Bahiru M; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA.; Program in Neuroscience and Behavior, University of Massachusetts, Amherst, MA 01003, USA., Stephens AD; Biology Department, University of Massachusetts Amherst, Amherst, MA. 01003, USA.; Molecular and Cellular Biology, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Jazyk:	angličtina
Zdroj:	Journal of cell science [J Cell Sci] 2024 Nov 06. Date of Electronic Publication: 2024 Nov 06.
DOI:	10.1242/jcs.262082
Abstrakt:	Nuclear blebs are herniations of the nucleus that occur in diseased nuclei that cause nuclear rupture leading to cellular dysfunction. Chromatin and lamins are two of the major structural components of the nucleus that maintain its shape and function, but their relative roles in nuclear blebbing remain elusive. To determine the composition of nuclear blebs, we compared the immunofluorescence intensity of DNA and lamin B in the main nucleus body to the nuclear bleb across cell types and perturbations. DNA density in the nuclear bleb was consistently decreased to about half of the nuclear body while lamin B levels in the nuclear bleb varied widely. Partial Wave Spectroscopic (PWS) microscopy recapitulated significantly decreased likelihood of high-density domains in the nuclear bleb versus body, independent of lamin B. Time lapse imaging into immunofluorescence reveals that decreased DNA density marks all nuclear blebs while decreased lamin B1 levels only occur in blebs that have recently ruptured. Thus, decreased DNA density is a better marker of a nuclear bleb than lamin B level. (© 2024. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu