Effects of Late-Passage Small Umbilical Cord-Derived Fast Proliferating Cells on Tenocytes from Degenerative Rotator Cuff Tears under an Interleukin 1β-Induced Tendinopathic Environment.

Autor: Lee AY; Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Dongjak-Gu, Seoul, 07061, Korea.; Institute of Reproductive Medicine and Population, Medical Research Center at, Seoul National University, Jongno-Gu, Seoul, 03087, Korea., Park JY; Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Dongjak-Gu, Seoul, 07061, Korea.; Department of Translational Medicine, Seoul National University College of Medicine, Jongno-Gu, Seoul, 03080, Korea., Hwang SJ; Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Dongjak-Gu, Seoul, 07061, Korea.; Department of Translational Medicine, Seoul National University College of Medicine, Jongno-Gu, Seoul, 03080, Korea., Jang KH; Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Dongjak-Gu, Seoul, 07061, Korea., Jo CH; Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Dongjak-Gu, Seoul, 07061, Korea. chrisjo@snu.ac.kr.; Institute of Reproductive Medicine and Population, Medical Research Center at, Seoul National University, Jongno-Gu, Seoul, 03087, Korea. chrisjo@snu.ac.kr.; Department of Translational Medicine, Seoul National University College of Medicine, Jongno-Gu, Seoul, 03080, Korea. chrisjo@snu.ac.kr.
Jazyk: angličtina
Zdroj: Tissue engineering and regenerative medicine [Tissue Eng Regen Med] 2024 Dec; Vol. 21 (8), pp. 1217-1231. Date of Electronic Publication: 2024 Nov 05.
DOI: 10.1007/s13770-024-00673-x
Abstrakt: Background: Tendinopathy is a chronic tendon disease. Mesenchymal stem cells (MSCs), known for their anti-inflammatory properties, may lose effectiveness with extensive culturing. Previous research introduced "small umbilical cord-derived fast proliferating cells" (smumf cells), isolated using a novel minimal cube explant method. These cells maintained their MSC characteristics through long-term culture. Thus, the purpose of the present study was to assess the anti-inflammatory effects of late-passage smumf cells at P10 on tenocytes derived from degenerative rotator cuff tears in a tendinopathic environment.
Methods: The mRNA expression with respect to aging of MSCs and secretion of growth factors (GFs) by smumf cells at P10 were measured. mRNA and protein synthesis in tenocytes with respect to the tenocyte phenotype, inflammatory cytokines, and matrix- degradation enzymes were measured. The inflammatory signal pathways involving nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) in tenocytes were also investigated. The proliferative response of degenerative tenocytes to co-culture with smumf cells over 7 days in varying IL-1β induced tendinopathic environments was investigated.
Results: smumf cells at P10 showed no signs of aging compared to those at P3. smumf cells at P10, secreting 2,043 pg/ml of hepatocyte growth factor (HGF), showed a 1.88-fold (p = .002) increase in HGF secretion in a tendinopathic environment. Degenerative tenocytes co-cultured with smumf cells showed significantly increased protein expression levels of collagen type I (Col I) and the Col I/III ratio by 1.46-fold (p < .001) and 1.66-fold (p < .001), respectively. The smumf cells at P10 reduced both mRNA and protein expression levels of matrix metalloproteinases-1, -2, -3, -8, -9, and -13 in tenocytes and attenuated NF-κB (phosphorylated IκBα/IκBα and phosphorylated p65/p65) and MAPK (phosphorylated p38/p38 and phosphorylated JNK/JNK) pathways activated by IL-1β. Removal of IL-1β from the co-culture accelerated the growth of tenocytes by 1.42-fold (p < .001). Removal of IL-1β accelerated tenocyte growth in co-cultures.
Conculsion: Late-passage smumf cells exert anti-inflammatory effects on tenocytes derived from degenerative rotator cuff tears under a tendinopathic environment, primarily through the secretion of growth factors (GFs).
Competing Interests: Declarations. Conflict of interest: CHJ owns shares of AcesoStem Biostrategies Inc. The authors declare that they have no competing interests. Ethical approval: The study was approved by the Seoul Metropolitan Government Seoul National University Boramae Medical Center Institutional Review Board (IRB No. 06–2012-78). Informed Consent was obtained from all patients before performing the study.
(© 2024. The Author(s).)
Databáze: MEDLINE
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