Standardization and evaluation of an in-house ELISA for the detection of rabies antibody in a tertiary care centre in South India.

Autor: Thangavelu K; Department of Clinical Virology, Christian Medical College, Vellore 632004, India., Daniel HD; Department of Clinical Virology, Christian Medical College, Vellore 632004, India., Kannangai R; Department of Clinical Virology, Christian Medical College, Vellore 632004, India., Abraham AM; Department of Clinical Virology, Christian Medical College, Vellore 632004, India., Velladurai SK; Department of Clinical Virology, Christian Medical College, Vellore 632004, India., Mammen DS; Department of Clinical Virology, Christian Medical College, Vellore 632004, India. Electronic address: shobamammen@cmcvellore.ac.in.
Jazyk: angličtina
Zdroj: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2024 Dec; Vol. 175, pp. 105742. Date of Electronic Publication: 2024 Oct 28.
DOI: 10.1016/j.jcv.2024.105742
Abstrakt: Background: Rapid fluorescent focus inhibition test (RFFIT), a neutralization-based assay for detecting rabies antibodies, is the gold standard. The National Action Plan for Dog Mediated Rabies Elimination (NAPRE) is a national program that strategizes the establishment of enzyme-linked immunosorbent assays (ELISA) to detect rabies antibodies.
Objective: We developed an in-house ELISA to screen for rabies antibodies using rabies vaccine antigen to study vaccine response among health care workers (HCWs) who received pre-exposure prophylaxis and a few animal bite victims who received post-exposure prophylaxis with rabies vaccine.
Study Design: A prospective study was carried out from April to September 2023 at the Department of Clinical Virology of a tertiary care center in South India. A total of 161 serum specimens, which included 155 serum samples from study participants and 6 samples from a reference laboratory (as controls), were obtained during the study period. Rabies antibody was determined by the in-house standardized ELISA developed using the rabies vaccine and compared with the reference assay, RFFIT. The accuracy indices of the in-house ELISA were estimated by MedCalc software (version 22.023).
Results: A panel of 86 positive and 75 negative serum samples was used for evaluating the in-house standardized ELISA. The sensitivity, specificity, positive and negative predictive values of the in-house ELISA were 98.8 %, 100 %, 100 %, and 98.7 % respectively. The accuracy of the in-house ELISA is 99.4 %.
Conclusion: ELISA can be a practically feasible and less expensive assay compared to RFFIT which is a cumbersome procedure with a long turn-around time of 3-4 days.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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