Precision Cut Lung Slices: Emerging Tools for Preclinical and Translational Lung Research. An Official American Thoracic Society Workshop Report.

Autor: Lehmann M; Philipps University Marburg, Institute for Lung Research, Marburg, Germany.; Helmholtz Center Munich, Institute for Lung Health and Immunity, Munich, Germany; mareike.lehmann@uni-marburg.de., Krishnan R; Beth Israel Deaconess Medical Center, Emergency Medicine, Boston, United States., Sucre J; Vanderbilt University Medical Center, Pediatrics, Nashville, Tennessee, United States., Kulkarni HS; Washington University in Saint Louis, Division of Pulmonary and Critical Care Medicine, Saint Louis, Missouri, United States., Pineda RH; University of Pittsburgh, Division of Pulmonary, Allergy and Critical Care Medicine, Pittsburgh, Pennsylvania, United States., Anderson C; University of Rochester, Pediatrics, Rochester, New York, United States., Banovich NE; Translational Genomics Research Institute, Phoenix, Arizona, United States., Behrsing HP; Institute for In Vitro Sciences Inc, Gaithersburg, Maryland, United States., Dean CH; Imperial College, National Heart and Lung Institute, London, United Kingdom of Great Britain and Northern Ireland., Haak A; Mayo Clinic College of Medicine, Rochester, Minnesota, United States., Gosens R; University of Groningen, Molecular Pharmacology, Groningen, Netherlands., Kaminski N; Yale School of Medicine , Pulmonary, Critical Care and Sleep Mediine , New Haven, Connecticut, United States., Zagorska A; Gilead Sciences Inc, Foster City, California, United States., Koziol-White C; Rutgers Institute for Translational Medicine and Science, Child Health Institute, Rutgers University, New Brunswick, New Jersey, United States., Metcalf JP; The University of Oklahoma Health Sciences Center, Medicine, Oklahoma City, Oklahoma, United States., Kim YH; U.S. Environmental Protection Agency, Research Triangle Park, North Carolina, United States., Loebel C; University of Michigan, USA, Ann Arbor, United States., Neptune E; Johns Hopkins, Medicine/Pulmonary and Critical Care, Baltimore, Maryland, United States., Noel A; Louisiana State University, Baton Rouge, Louisiana, United States., Raghu G; University of Washington Medical Center, Division of Pulmonary and Critical Care Medicine, Seattle, Washington, United States., Sewald K; Fraunhofer ITEM, Airway Immunology, Hannover, Germany., Sharma A; University of Florida, Gainesville, Florida, United States., Suki B; Boston University, Biomedical Engineering, Boston, Massachusetts, United States., Sperling A; University of Virginia School of Medicine, Charlottesville, Virginia, United States., Tatler A; University of Nottingham, Respiratory Medicine , Nottingham, United Kingdom of Great Britain and Northern Ireland., Turner S; Pliant Therapeutics, South San Francisco, California, United States., Rosas IO; Brigham and Women's Hospital, Department of Medicine, Division of Pulmonary and Critical Care Medicine, Boston, Massachusetts, United States., van Ry P; Brigham Young University, Chemistry and Biochemistry, Provo, Utah, United States., Wille T; Bundeswehr Institute of Pharmacology and Toxicology, Bundeswehr Medical Academy, Germany, Munich, Germany., Randell SH; University of North Carolina, Department of Cell Biology & Physiology, Chapel Hill, North Carolina, United States., Pryhuber G; University of Rochester, Pediatrics, Rochester, New York, United States., Rojas M; Ohio State University, Columbus, OH, Pulmonary, Critical Care and Sleep Medicine, College of Medicine, , Columbus, Ohio, United States., Bourke J; Monash University, Department of Pharmacology, Biomedicine Discovery Institute, Clayton, Victoria, Australia., Königshoff M; University of Pittsburgh, Medicine, Pittsburgh, Pennsylvania, United States.
Jazyk: angličtina
Zdroj: American journal of respiratory cell and molecular biology [Am J Respir Cell Mol Biol] 2024 Nov 05. Date of Electronic Publication: 2024 Nov 05.
DOI: 10.1165/rcmb.2024-0479ST
Abstrakt: The urgent need for effective treatments for acute and chronic lung diseases underscores the significance of developing innovative preclinical human research tools. The 2023 ATS Workshop on Precision Cut Lung Slices (PCLS) brought together 35 experts to discuss and address the role of human tissue-derived PCLS as a unique tool for target and drug discovery and validation in pulmonary medicine. With increasing interest and usage, along with advancements in methods and technology, there is a growing need for consensus on PCLS methodology and readouts. The current document recommends standard reporting criteria and emphasizes the requirement for careful collection and integration of clinical metadata. We further discuss current clinically relevant readouts that can be applied to PCLS and highlight recent developments and future steps for implementing novel technologies for PCLS modeling and analysis. The collection and correlation of clinical metadata and multiomic analysis will further advent the integration of this preclinical platform into patient endotyping and the development of tailored therapies for lung disease patients.
Databáze: MEDLINE