Low-cost high-throughput targeted sequencing for the accurate detection of respiratory tract pathogens.
| Autor: | Ju C; Nanshan Center for Disease Control and Prevention, Shenzhen, China., Zhou C; Nanan Center for Disease Control and Prevention, Chongqing, China., Deng Z; The First Affiliated Hospital of Sun Yue-sen University, Guangzhou, China., Gao J; Guangdong Ardent BioMed Co., Ltd, Guangzhou, China., Jiang W; Guangdong Ardent BioMed Co., Ltd, Guangzhou, China., Zeng H; Guangdong Ardent BioMed Co., Ltd, Guangzhou, China., Huang H; The First Affiliated Hospital of Sun Yue-sen University, Guangzhou, China., Duan Y; Nanshan Center for Disease Control and Prevention, Shenzhen, China., Deng DX; Guangdong Ardent BioMed Co., Ltd, Guangzhou, China. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of infection in developing countries [J Infect Dev Ctries] 2024 Sep 30; Vol. 18 (9.1), pp. S50-S55. Date of Electronic Publication: 2024 Sep 30. |
| DOI: | 10.3855/jidc.19685 |
| Abstrakt: | Introduction: The current gold standard for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnosis by real-time reverse transcriptase polymerase chain reaction (RT-PCR) is limited by the number of genes that can be detected. In this study, we developed a low-cost and high-throughput next-generation sequencing technology that can overcome the limitations of real time RT-PCR. Methodology: A targeted sequencing panel (TSP) consisting of approximately 500 amplicons was designed. This panel could simultaneously detect a broad range of gene loci of SARS-CoV-2, and genes for the most common infectious viruses that affect the respiratory tract, in a single run and could include up to 96 samples. Four hundred and forty-eight samples and 31 control samples were analyzed independently with both TSP and RT-PCR, and the results were compared for accuracy and other indicators. Results: TSP identified 50 SARS-CoV-2 positive samples with a 99.33% match to RT-PCR results. It is not surprising that TSP also identified multiple infections from the 96 samples, whereas RT-PCR could not. Thus, TSP was able to accurately diagnose the samples which could not be identified based on single RT-PCR test. Conclusions: Our data demonstrated that TSP is a fast and accurate testing method for identifying multiple pathogen infections of the respiratory tract. Competing Interests: No Conflict of Interest is declared (Copyright (c) 2024 Changyan Ju, Chengbosen Zhou, Zhezhi Deng, Jingwei Gao, Weizhao Jiang, Hanbing Zeng, Haiwei Huang, Yongxiang Duan, David X Deng.) |
| Databáze: | MEDLINE |
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