Transcriptomic analysis of the 12 major human breast cell types reveals mechanisms of cell and tissue function.

Autor: Del Toro K; Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, University of New Mexico, Albuquerque, New Mexico, United States of America., Sayaman R; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America., Thi K; Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America., Licon-Munoz Y; Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, University of New Mexico, Albuquerque, New Mexico, United States of America., Hines WC; Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, University of New Mexico, Albuquerque, New Mexico, United States of America.
Jazyk: angličtina
Zdroj: PLoS biology [PLoS Biol] 2024 Nov 05; Vol. 22 (11), pp. e3002820. Date of Electronic Publication: 2024 Nov 05 (Print Publication: 2024).
DOI: 10.1371/journal.pbio.3002820
Abstrakt: A fundamental question in biology, central to our understanding of cancer and other pathologies, is determining how different cell types coordinate to form and maintain tissues. Recognizing the distinct features and capabilities of the cells that compose these tissues is critical. Unfortunately, the complexity of tissues often hinders our ability to distinguish between neighboring cell types and, in turn, scrutinize their transcriptomes and generate reliable and tractable cell models for studying their inherently different biologies. We have recently introduced a novel method that permits the identification and purification of the 12 cell types that compose the human breast-nearly all of which could be reliably propagated in the laboratory. Here, we explore the nature of these cell types. We sequence mRNAs from each purified population and investigate transcriptional patterns that reveal their distinguishing features. We describe the differentially expressed genes and enriched biological pathways that capture the essence of each cell type, and we highlight transcripts that display intriguing expression patterns. These data, analytic tools, and transcriptional analyses form a rich resource whose exploration provides remarkable insights into the inner workings of the cell types composing the breast, thus furthering our understanding of the rules governing normal cell and tissue function.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Del Toro et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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