Quorum sensing orchestrates parallel cell death pathways in Vibrio cholerae via Type 6 secretion-dependent and -independent mechanisms.

Autor:	Mashruwala AA; Department of Molecular Biology, Princeton University, Princeton, NJ 08544.; HHMI, Chevy Chase, MD 20815., Bassler BL; Department of Molecular Biology, Princeton University, Princeton, NJ 08544.; HHMI, Chevy Chase, MD 20815.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Nov 12; Vol. 121 (46), pp. e2412642121. Date of Electronic Publication: 2024 Nov 05.
DOI:	10.1073/pnas.2412642121
Abstrakt:	Quorum sensing (QS) is a cell-to-cell communication process that enables bacteria to coordinate group behaviors. In Vibrio cholerae colonies, a program of spatial-temporal cell death is among the QS-controlled traits. Cell death occurs in two phases, first along the colony rim, and subsequently, at the colony center. Both cell death phases are driven by the type 6 secretion system (T6SS). Here, we show that HapR, the master QS regulator, does not control t6ss gene expression nor T6SS-mediated killing activity. Nonetheless, a Δ hapR strain displays no cell death at the colony rim. RNA-Sequencing (RNA-Seq) analyses reveal that HapR activates expression of an operon containing four genes of unknown function, vca0646-0649. Epistasis and overexpression studies show that two of the genes, vca0646 and vca0647 , are required to drive cell death in both a Δ hapR and a Δ hapR Δ t6ss strain. Thus, vca0646 - 0649 are regulated by HapR but act independently of the T6SS machinery to cause cell death, suggesting that a second, parallel pathway to cell death exists in V. cholerae . Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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