Mitochondrial respiratory pathways in immature rat heart tissue using different cardioplegic solutions.

Autor: Mamedov A; Department of Cardiac, Thoracic and Vascular Surgery, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania., Rumbinaitė E; Department of Cardiology, of Medicine, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania., Romann S; Department of Internal Medicine III: Cardiology, Angiology & Pulmonology, Heidelberg University Hospital, Heidelberg, Germany., Verikas D; Department of Cardiology, of Medicine, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania., Jakuška P; Department of Cardiac, Thoracic and Vascular Surgery, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania., Aitaliyev S; Department of Cardiac, Thoracic and Vascular Surgery, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania. aitaliyev.serik@gmail.com.; Faculty of Medicine and Health Care, Al-Farabi Kazakh National University, Almaty, Kazakhstan. aitaliyev.serik@gmail.com., Benetis R; Department of Cardiac, Thoracic and Vascular Surgery, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania., Stankevičius E; Institute of Physiology and Pharmacology, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Jazyk: angličtina
Zdroj: General thoracic and cardiovascular surgery [Gen Thorac Cardiovasc Surg] 2024 Nov 05. Date of Electronic Publication: 2024 Nov 05.
DOI: 10.1007/s11748-024-02097-9
Abstrakt: Introduction: Minor defects in the mitochondrial ATP-generating system and post-cardioplegia oxidative phosphorylation can negatively impact cardiac function in immature hearts. This study aimed to examine the mitochondrial respiratory pathway using three different cardioplegic solutions (Custodiol HTK, St. Thomas, and Del Nido) during moderate (1 h) and long (3 h) ischemic periods.
Methods: A total of 41 male Wistar albino rats were utilized in this study. Five experiments were conducted without the use of any cardioplegic solution (CP0 group). To assess both moderate and prolonged ischemic periods, six experiments were carried out in each of the following groups: CP1 group (St. Thomas solution), CP2 group (Custodiol HTK solution), and CP3 group (Del Nido solution).
Results: After 1 h, the highest mitochondrial respiration rate was observed in the CP3 group and the lowest in the CP1 group (p = 0.006). After adding ADP substrate, the highest mitochondrial ATP-production-coupled respiration was recorded in the CP3 group, which was similar to the control group CP0. After 3 h, while evaluating the ratio between mitochondrial respiration ATP-production coupled and basal respiration, significant differences were found between CP1 group and CP3 group (p = 0.035), as well as between the CP1 and CP0 groups (p = 0.045). Additionally, by assessing the condition of the outer mitochondrial membrane using the Cyt C effect (Cyt/Phos [ADP]), significant differences were observed between the CP1 and CP3 group (p = 0.004), as well as between CP1 and CP0 groups (p = 0.003).
Conclusion: Del Nido cardioplegic solution provided optimal mitochondrial protection under moderate and long myocardial ischemia conditions.
Competing Interests: Declarations. Conflict of interests: The authors stated that they have no conflicts of interest regarding the research, writing, or publication of this article.
(© 2024. The Author(s), under exclusive licence to The Japanese Association for Thoracic Surgery.)
Databáze: MEDLINE
Externí odkaz: