RNA Extraction from Rice Immature Embryo Using Laser Capture Microdissection.

Autor:	Tezuka T; National Institute of Genetics, Mishima, Shizuoka, Japan. takutezuka@nig.ac.jp., Nosaka-Takahashi M; National Institute of Genetics, Mishima, Shizuoka, Japan., Yoshikawa T; National Institute of Genetics, Mishima, Shizuoka, Japan., Sato Y; National Institute of Genetics, Mishima, Shizuoka, Japan.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2869, pp. 21-27.
DOI:	10.1007/978-1-0716-4204-7_4
Abstrakt:	Laser capture microdissection (LCM) enables the selective isolation of organs, tissues, and cells from surrounding tissues. Total RNA extracted from small tissue sections can be used for a variety of subsequent analysis such as RNA-seq analysis. Here, we describe a method for isolating embryos from rice ovary sections using LCM and extracting total RNA. We successfully obtained sufficient amount of total RNA from a single rice embryo at the globular embryo stage for RNA-seq analysis. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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