| Autor: |
Habib L; Interdisciplinary Unit: Lipids, Analytical and Biological Systems Lip(Sys)2, Faculty of Pharmacy, Université Paris-Saclay, F-91400 Orsay, France. rime.michael-jubeli@universite-paris-saclay.fr.; Laboratoire d'étude cinétique en milieu hétérogène (LECH), Saint Joseph University, Campus des Sciences et Technologies, Mar Roukos, Mkallès, P.O Box 11-514, Riad El Solh, 1107 2050 Beirut, Lebanon., Michael-Jubeli R; Interdisciplinary Unit: Lipids, Analytical and Biological Systems Lip(Sys)2, Faculty of Pharmacy, Université Paris-Saclay, F-91400 Orsay, France. rime.michael-jubeli@universite-paris-saclay.fr., Abboud M; Physics department, UR TVA, Faculty of science, Saint Joseph University, B.P. 11-514-Riad El Solh, 1107 2050 Beirut, Lebanon., Lteif R; Laboratoire d'étude cinétique en milieu hétérogène (LECH), Saint Joseph University, Campus des Sciences et Technologies, Mar Roukos, Mkallès, P.O Box 11-514, Riad El Solh, 1107 2050 Beirut, Lebanon., Tfayli A; Interdisciplinary Unit: Lipids, Analytical and Biological Systems Lip(Sys)2, Faculty of Pharmacy, Université Paris-Saclay, F-91400 Orsay, France. rime.michael-jubeli@universite-paris-saclay.fr. |
| Abstrakt: |
Skin health relies heavily on a well-maintained cutaneous barrier. While the detrimental effects of UV radiation on the epidermis are established, the impact of blue light, a significant component of sunlight and artificial sources, is less clear. This study aims to explore blue light's influence on the reconstructed human epidermis (RHE) using two complementary analytical approaches: Raman microspectroscopy and normal phase liquid chromatography coupled with high-resolution mass spectrometry (NPLC/HR-MS n ). RHE samples were exposed to blue light (415 nm and 455 nm) during different stages of their maturation. Raman spectra were acquired for both irradiated and non-irradiated (control) samples. Raman descriptors were analyzed to assess potential alterations in the structural organization of proteins and lipids' conformational changes. Additionally, lipids from RHE samples were extracted and analyzed using NPLC/HR-MS n . Blue light exposure led to changes in the structural organization of RHE lipids and proteins, as well as changes in the lipid composition. These changes varied depending on the wavelength and exposure dose. Exposure to blue light could disrupt the integrity of the skin's protective barrier, leading to increased sensitivity to environmental stressors and potential skin damage. |
