Comparative analysis of the leprosy detection rate regarding its clinical spectrum through PCR using the 16S rRNA gene: a scientometrics and meta-analysis.
| Autor: | Silva MJA; Ph.D and Master Program in Parasitic Biology in the Amazon (PPGBPA), State University of Pará (UEPA), Belém, Pará, Brazil.; Bacteriology and Mycology Section (SABMI), Evandro Chagas Institute (IEC), Ananindeua, Pará, Brazil.; Universidade Tecnológica Federal do Paraná (UTFPR), Dois Vizinhos, Paraná, Brazil., Brasil TP; Faculty of Medicine, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil., Silva CS; Ph.D and Master Program in Parasitic Biology in the Amazon (PPGBPA), State University of Pará (UEPA), Belém, Pará, Brazil., Frota CC; Faculty of Medicine, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil., Sardinha DM; Ph.D and Master Program in Parasitic Biology in the Amazon (PPGBPA), State University of Pará (UEPA), Belém, Pará, Brazil., Figueira LRT; Ph.D and Master Program in Parasitic Biology in the Amazon (PPGBPA), State University of Pará (UEPA), Belém, Pará, Brazil., Neves KAS; Ph.D and Master Program in Parasitic Biology in the Amazon (PPGBPA), State University of Pará (UEPA), Belém, Pará, Brazil., Dos Santos EC; Bacteriology and Mycology Section (SABMI), Evandro Chagas Institute (IEC), Ananindeua, Pará, Brazil., Lima KVB; Bacteriology and Mycology Section (SABMI), Evandro Chagas Institute (IEC), Ananindeua, Pará, Brazil., Ghisi NC; Programa de Pós-Graduação em Biotecnologia, Universidade Tecnológica Federal do Paraná (UTFPR), Dois Vizinhos, Paraná, Brazil., Lima LNGC; Bacteriology and Mycology Section (SABMI), Evandro Chagas Institute (IEC), Ananindeua, Pará, Brazil. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Frontiers in microbiology [Front Microbiol] 2024 Oct 21; Vol. 15, pp. 1497319. Date of Electronic Publication: 2024 Oct 21 (Print Publication: 2024). |
| DOI: | 10.3389/fmicb.2024.1497319 |
| Abstrakt: | Background: Leprosy is a chronic and disabling infectious disease caused by Mycobacterium leprae . It has a wide clinical spectrum and is operationally classified into paucibacillary (PB) and multibacillary (MB) cases. There is evidence that the 16S rRNA gene can be used in Polymerase Chain Reaction (PCR) for complementary detection with high sensitivity and specificity. However, there is no literature convention on its diagnostic correspondence regarding the particular operational classification of the disease. This study aimed to correlate, through a meta-analysis, the detection rate of leprosy between the PCR method with the 16S rRNA gene in the clinical forms PB and MB in relation to confirmed cases. Methods: This is a systematic review and meta-analysis study conducted according to the PRISMA 2020 guidelines, using the search descriptors with "AND": "Leprosy"; "Polymerase Chain Reaction"; " 16S rRNA " in the PUBMED, SciELO, LILACS, and Science Direct databases. The search was limited to original observational articles in Portuguese, English, or Spanish, with no defined time frame. The methodological quality assessment of the selected articles was performed using the JBI checklists. A scientometric approach to the article using used the VOS Viewer and Scimago Graphica software. The meta-analysis was conducted using Comprehensive Meta-Analyses software, under Pearson's Correlation effect test and fixed effect model and subgroup analysis concerning the type of sample analyzed. Results: The study was significant from the perspective of the paucibacillary group (Clinical biopsy: -0.45 [95% CI= -0.63 - -0.22], p < 0.001/ Slit smear skin: -0.52 [95% CI= -0.65 - -0.36], p < 0.001 / Overall: -0.50 [95% CI= -0.61 - -0.37], p < 0.001). The PCR diagnostic method for the16S rRNAgene ofM. lepraehas low viability and diagnostic sensitivity in both clinical biopsy samples and leprosy skin smears. Conclusion: This implies little validation of it as a PCR target gene for diagnosing the disease, highlighting limitations in the actual technique. Systematic Review Registration: https://www.crd.york.ac.uk/prospero/, identifier CRD42024588790. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Silva, Brasil, Silva, Frota, Sardinha, Figueira, Neves, dos Santos, Lima, Ghisi and Lima.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|