Clarification of a unique mucosal vaccination route for improved systemic and mucosal immune response in broiler.

Autor:	Akter R; Department of Anatomy, Histology & Physiology, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh., Masum MA; Department of Anatomy, Histology & Physiology, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh., Biswas S; Department of Anatomy, Histology & Physiology, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh., Rubel MZU; Department of Poultry Science, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh., Sarkar SK; Department of Anatomy, Histology & Physiology, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh., Islam MS; Department of Anatomy, Histology & Physiology, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh., Golbar HM; Department of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, 6205, Bangladesh., Alam ME; Department of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, 6205, Bangladesh., Rakib MA; Quality Assurance, Beximco Pharmaceuticals Limited, Dhaka, 1700, Bangladesh., Isalm Khan MZ; Department of Pre-Clinical courses, Gono Bishwabidyalay, Dhaka, 1344, Bangladesh.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Oct 19; Vol. 10 (20), pp. e39621. Date of Electronic Publication: 2024 Oct 19 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e39621
Abstrakt:	There are often outbreaks of infectious diseases on farms, which not only disrupt production but also cause significant economic losses. Vaccines are given to prevent the spread of these infectious diseases, but they produce only systemic antibodies or antibodies in the mucosa of a particular system. So, a new mucosal vaccination route is needed where the vaccine will produce antibodies in the systemic circulation as well as in the mucosa of many systems. In our study, the cloaca was targeted because it is associated with the mucosa of many systems. Whole-mount and routine histological staining show both lymphatic nodules and diffuse lymphatic tissues in the lamina propria of cloaca. These lymphatic tissues are made up of Bu-1+ B-cells, CD3 + T-cells, and KUL01+ macrophages and monocytes. So, this is a new mucosa-associated lymphoid tissue, named cloaca-associated lymphoid tissue (CALT). The CALT contains antigen-presenting cells (dendritic cells, macrophages, B cells, MHC II molecules, and T cells) and is equipped with blood vessels and high endothelial venules, which indicate its functional status. More antibodies were produced in the treatment group compared to the vehicle control group after administration of the infectious bursal disease (IBD ) and the Newcastle disease (ND) vaccine through cloaca. In addition, the cloaca-associated route produces a higher number of antibodies than the other traditional routes, which reveals the uniqueness of this route. Cloacal-vaccinated chickens showed less damage to the follicle and epithelium of the bursa of Fabricius compared to other groups, indicating its lower cytotoxic effect. Therefore, the cloaca-associated mucosal vaccination route produces more antibodies than other mucosal vaccination routes, which will protect the chickens on the farm to a greater extent. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Md. Abdul Masum reports financial support was provided by University Grants Commission of Bangladesh. Md. Abdul Masum reports financial support was provided by Government of the People's Republic of Bangladesh Ministry of Science and Technology. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors. Published by Elsevier Ltd.)
Databáze:	MEDLINE
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