Leveraging deep single-soma RNA sequencing to explore the neural basis of human somatosensation.

Autor: Yu H; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Nagi SS; Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden., Usoskin D; Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology, Karolinska Institute, Stockholm, Sweden., Hu Y; Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology, Karolinska Institute, Stockholm, Sweden., Kupari J; Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology, Karolinska Institute, Stockholm, Sweden., Bouchatta O; Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden., Yan H; Department of Biostatistics in Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Cranfill SL; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Gautam M; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Su Y; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Lu Y; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Wymer J; Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA., Glanz M; Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA., Albrecht P; Neuroscience & Pain Research Group, Integrated Tissue Dynamics, LLC, Rensselaer, NY, USA., Song H; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Ming GL; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Prouty S; Department of Dermatology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Seykora J; Department of Dermatology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Wu H; Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Ma M; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Marshall A; Pain Research Institute, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK., Rice FL; Neuroscience & Pain Research Group, Integrated Tissue Dynamics, LLC, Rensselaer, NY, USA., Li M; Department of Biostatistics in Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Olausson H; Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden. hakan.olausson@liu.se., Ernfors P; Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology, Karolinska Institute, Stockholm, Sweden. patrik.ernfors@ki.se., Luo W; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. luow@pennmedicine.upenn.edu.
Jazyk: angličtina
Zdroj: Nature neuroscience [Nat Neurosci] 2024 Dec; Vol. 27 (12), pp. 2326-2340. Date of Electronic Publication: 2024 Nov 04.
DOI: 10.1038/s41593-024-01794-1
Abstrakt: The versatility of somatosensation arises from heterogeneous dorsal root ganglion (DRG) neurons. However, soma transcriptomes of individual human (h)DRG neurons-critical information to decipher their functions-are lacking due to technical difficulties. In this study, we isolated somata from individual hDRG neurons and conducted deep RNA sequencing (RNA-seq) to detect, on average, over 9,000 unique genes per neuron, and we identified 16 neuronal types. These results were corroborated and validated by spatial transcriptomics and RNAscope in situ hybridization. Cross-species analyses revealed divergence among potential pain-sensing neurons and the likely existence of human-specific neuronal types. Molecular-profile-informed microneurography recordings revealed temperature-sensing properties across human sensory afferent types. In summary, by employing single-soma deep RNA-seq and spatial transcriptomics, we generated an hDRG neuron atlas, which provides insights into human somatosensory physiology and serves as a foundation for translational work.
Competing Interests: Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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