Genome-guided isolation of the hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota.

Autor: Nou NO; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA., Covington JK; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA., Lai D; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA., Mayali X; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Seymour CO; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA., Johnston J; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Jiao JY; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory, Sun Yat-Sen University, Zhuhai, PR China.; School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China., Buessecker S; Department of Earth System Science, Stanford University, Stanford, CA, USA., Mosier D; Department of Biology, California State University, San Bernardino, CA, USA.; Department of Earth, Energy, and Environment, University of Calgary, Calgary, AB, Canada., Muok AR; Department of Microbial Sciences, Institute of Biology, Leiden University, Sylviusweg 72, Leiden, The Netherlands., Torosian N; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA., Cook AM; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA., Briegel A; Department of Microbial Sciences, Institute of Biology, Leiden University, Sylviusweg 72, Leiden, The Netherlands., Woyke T; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; University of California Merced, Life and Environmental Sciences, Merced, CA, USA., Eloe-Fadrosh E; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Shapiro N; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Bryan SG; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA., Sleezer S; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA., Dimapilis J; Department of Biology, California State University, San Bernardino, CA, USA., Gonzalez C; Department of Biology, California State University, San Bernardino, CA, USA., Gonzalez L; Department of Biology, California State University, San Bernardino, CA, USA., Noriega M; Department of Biology, California State University, San Bernardino, CA, USA., Hess M; Department of Animal Science, University of California Davis, Davis, CA, USA., Carlson RP; Chemical and Biological Engineering, Montana State University, Bozeman, MT, USA.; Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA., Liu L; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory, Sun Yat-Sen University, Zhuhai, PR China.; School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China., Li MM; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory, Sun Yat-Sen University, Zhuhai, PR China.; School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China., Lian ZH; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory, Sun Yat-Sen University, Zhuhai, PR China.; School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China., Zhu S; CAS Key Laboratory of Tropical Marine Bio Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Guangdong Provincial Observation and Research Station for Coastal Upwelling Ecosystem, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China., Liu F; CAS Key Laboratory of Tropical Marine Bio Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Guangdong Provincial Observation and Research Station for Coastal Upwelling Ecosystem, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; University of Chinese Academy of Sciences, Beijing, PR China., Sun X; CAS Key Laboratory of Tropical Marine Bio Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Guangdong Provincial Observation and Research Station for Coastal Upwelling Ecosystem, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China., Gao B; CAS Key Laboratory of Tropical Marine Bio Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China.; Guangdong Provincial Observation and Research Station for Coastal Upwelling Ecosystem, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, PR China., Mewalal R; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Harmon-Smith M; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Blaby IK; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Cheng JF; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Weber PK; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Grigorean G; Proteomics Core, University of California Davis, Davis, CA, USA., Li WJ; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory, Sun Yat-Sen University, Zhuhai, PR China.; School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.; State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, PR China., Dekas AE; Department of Earth System Science, Stanford University, Stanford, CA, USA., Pett-Ridge J; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA., Dodsworth JA; Department of Biology, California State University, San Bernardino, CA, USA., Palmer M; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA. marike.palmer@umanitoba.ca.; Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada. marike.palmer@umanitoba.ca., Hedlund BP; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV, USA. brian.hedlund@unlv.edu.; Nevada Institute of Personalized Medicine, University of Nevada Las Vegas, Las Vegas, NV, USA. brian.hedlund@unlv.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Nov 04; Vol. 15 (1), pp. 9534. Date of Electronic Publication: 2024 Nov 04.
DOI: 10.1038/s41467-024-53784-3
Abstrakt: Few aerobic hyperthermophilic microorganisms degrade polysaccharides. Here, we describe the genome-enabled enrichment and optical tweezer-based isolation of an aerobic polysaccharide-degrading hyperthermophile, Fervidibacter sacchari, previously ascribed to candidate phylum Fervidibacteria. F. sacchari uses polysaccharides and monosaccharides for growth at 65-87.5 °C and expresses 191 carbohydrate-active enzymes (CAZymes) according to RNA-Seq and proteomics, including 31 with unusual glycoside hydrolase domains (GH109, GH177, GH179). Fluorescence in-situ hybridization and nanoscale secondary ion mass spectrometry confirmed rapid assimilation of 13 C-starch in spring sediments. Purified GHs were optimally active at 80-100 °C on ten different polysaccharides. Finally, we propose reassigning Fervidibacteria as a class within phylum Armatimonadota, along with 18 other species, and show that a high number and diversity of CAZymes is a hallmark of the phylum, in both aerobic and anaerobic lineages. Our study establishes Fervidibacteria as hyperthermophilic polysaccharide degraders in terrestrial geothermal springs and suggests a broad role for Armatimonadota in polysaccharide catabolism.
(© 2024. The Author(s).)
Databáze: MEDLINE
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