Identification and characterization of the lipoprotein N -acyltransferase in Bacteroides .

Autor:	Armbruster KM; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109., Jiang J; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109., Sartorio MG; Department of Molecular Microbiology, Washington University School of Medicine, Saint Louis, MO 63110., Scott NE; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, VIC 3000, Australia., Peterson JM; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109., Sexton JZ; Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109.; Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109., Feldman MF; Department of Molecular Microbiology, Washington University School of Medicine, Saint Louis, MO 63110., Koropatkin NM; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Nov 12; Vol. 121 (46), pp. e2410909121. Date of Electronic Publication: 2024 Nov 04.
DOI:	10.1073/pnas.2410909121
Abstrakt:	Members of the Bacteroidota compose a large portion of the human gut microbiota, contributing to overall gut health via the degradation of various polysaccharides. This process is facilitated by lipoproteins, globular proteins anchored to the cell surface by a lipidated N-terminal cysteine. Despite their importance, lipoprotein synthesis by these bacteria is understudied. In Escherichia coli , the α-amino-linked lipid of lipoproteins is added by the l ipoprotein N -acyl t ransferase Lnt. Herein, we have identified a protein distinct from Lnt responsible for the same process in Bacteroides , named l ipoprotein N -acyltransferase in B acteroides (Lnb). Deletion of Lnb yields cells that synthesize diacylated lipoproteins, with impacts on cell viability and morphology, growth on polysaccharides, and protein composition of membranes and outer membrane vesicles (OMVs). Our results not only challenge the accepted paradigms of lipoprotein biosynthesis in gram-negative bacteria but also suggest the existence of a new family of lipoprotein N -acyltransferases. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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