The role of vitamin D: a promising pathway to combat neuropsychiatric lupus disorders.

Autor:	Karnopp TE; Laboratory of Autoimmune Diseases, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Medical Sciences Postgraduate Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.; Division of Rheumatology, Department of Internal Medicine, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Chapacais GF; Laboratory of Autoimmune Diseases, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Medical Sciences Postgraduate Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.; Division of Rheumatology, Department of Internal Medicine, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Gasparini ML; Laboratory of Autoimmune Diseases, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Medical Sciences Postgraduate Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.; Division of Rheumatology, Department of Internal Medicine, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Dos Santos NG; Laboratory of Autoimmune Diseases, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Medical Sciences Postgraduate Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.; Division of Rheumatology, Department of Internal Medicine, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., da Silva Freitas V; Laboratory of Autoimmune Diseases, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Division of Rheumatology, Department of Internal Medicine, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Piccoli M; Laboratory of Autoimmune Diseases, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Division of Rheumatology, Department of Internal Medicine, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Di Domenico AL; Laboratory of Autoimmune Diseases, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Division of Rheumatology, Department of Internal Medicine, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Doria LD; Laboratory of Autoimmune Diseases, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Medical Sciences Postgraduate Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.; Division of Rheumatology, Department of Internal Medicine, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., de Souza NMP; Department of Life Sciences, University of Santa Cruz do Sul, Santa Cruz do Sul, RS Brazil., Rieger A; Department of Life Sciences, University of Santa Cruz do Sul, Santa Cruz do Sul, RS Brazil.; Graduate Program in Health Promotion, University of Santa Cruz do Sul, UNISC, Santa Cruz do Sul, RS, Brazil., Freitas EC; Laboratory of Autoimmune Diseases, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Division of Rheumatology, Department of Internal Medicine, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Visioli F; Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.; Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Monticielo OA; Laboratory of Autoimmune Diseases, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Division of Rheumatology, Department of Internal Medicine, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Jazyk:	angličtina
Zdroj:	Clinical and experimental immunology [Clin Exp Immunol] 2024 Nov 04. Date of Electronic Publication: 2024 Nov 04.
DOI:	10.1093/cei/uxae099
Abstrakt:	To evaluate neuropsychiatric manifestations in the pristane-induced lupus (PIL) model, as well as to evaluate immunoregulatory effects of vitamin D (vit-D) in the brain of mice with PIL. Eighty female BALB/c mice were divided into six groups with 90 (3 months) and 180 (6 months) days of experimentation: CO3, CO6 (controls), PIL3, PIL6 (pristane-induced lupus), VD3 and VD6 (PIL supplemented with 1,25-dihydroxyvitamin D). Forced-swim, elevated plus maze and Barnes maze were the behavioral tests performed. Expression of pVDR was assessed by immunofluorescence. Brain IgM and IgG deposits were evaluated by double staining fluorescence. Serum IL-6 and IFN-α1 were quantified by ELISA. AUC-ROC curve was also performed for immunoglobulins. PIL and VD showed depressive-like behavior in the forced-swim test and anxious-like behavior in the elevated plus maze test. PIL also presented cognitive and memory impairment in the Barnes maze test. Additionally, PIL and VD presented higher levels of serum IFN-α1, but not IL-6. Mice supplemented with vit-D had reduced IgM and IgG deposits and increased pVDR expression in the brain after 180 days. The AUC-ROC curve demonstrated high sensitivity and specificity for IgM and IgG in the brain. We observed neuropsychiatric manifestations in this model of systemic lupus erythematosus (SLE), strongly corroborating to PIL model being suitable as a neuropsychiatric lupus (NPSLE) model. Vit-D was able to reduce immunoglobulin deposits in the brain and influenced the levels of serum IL-6 in the animals assessed. Also, it improved memory, but it had no effect on depressive and anxious-like behavior. (© The Author(s) 2024. Published by Oxford University Press on behalf of the British Society for Immunology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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