Enhancing orofacial pain relief: α-phellandrene complexed with hydroxypropyl-β-cyclodextrin mitigates orofacial nociception in rodents.

Autor: Machado BG; Department of Physiology, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil., Passos FRS; Department of Physiology, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil., Antoniolli ÂR; Department of Physiology, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil., Menezes Pereira EW; Department of Physiology, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil., Santos TKB; Department of Physiology, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil., Monteiro BS; Department of Physiology, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil., de Souza Siqueira Lima P; Department of Odontology, Centro Universitário de Excelência (Unex), Feira de Santana, BA, Brazil., Matos SS; Department of Pharmacy, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil., Duarte MC; Department of Pharmacy, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil., de Souza Araújo AA; Department of Pharmacy, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil., da Silva Almeida JRG; Núcleo de Estudos E Pesquisas de Plantas Medicinais (Neplame), Federal University of San Francisco Valley, Petrolina, PE, Brazil., Oliveira Júnior RG; Faculty of Pharmacy, University Paris Cité (UPCité), Paris, France., Coutinho HDM; Department of Biological Chemistry, Regional University of Cariri (URCA), Crato, CE, Brazil. hdmcoutinho@gmail.com., Quintans-Júnior LJ; Department of Physiology, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil., de Souza Siqueira Quintans J; Department of Physiology, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil. jullyanaquintans@gmail.com.
Jazyk: angličtina
Zdroj: Naunyn-Schmiedeberg's archives of pharmacology [Naunyn Schmiedebergs Arch Pharmacol] 2024 Nov 04. Date of Electronic Publication: 2024 Nov 04.
DOI: 10.1007/s00210-024-03561-2
Abstrakt: Orofacial pain affects 10-15% of adults and can severely impact quality of life. Despite ongoing treatment challenges, monoterpene alpha-phellandrene (PHE) shows potential therapeutic benefits. This study aimed to develop and evaluate an inclusion complex of PHE with hydroxypropyl-beta-cyclodextrin (PHE-HPβCD) for treating orofacial pain. The PHE-HPβCD complex was created using physical mixing and characterized by differential scanning calorimetry (DSC) and high-performance liquid chromatography (HPLC) to determine encapsulation efficiency. The complex exhibited a 70.45% encapsulation efficiency. Male Swiss mice were used in models of orofacial pain induced by formalin, cinnamaldehyde, glutamate, and corneal nociception by hypertonic saline. Additionally, cytokine levels (TNF-α and IL-1β) were measured in the upper lip tissue of mice subjected to the formalin model. Both PHE and PHE-HPβCD showed significant antinociceptive effects at a 50 mg/kg dose during formalin-induced pain, reducing both neurogenic and inflammatory phases of pain. PHE-HPβCD also reduced TNF-α and IL-1β levels. For cinnamaldehyde and glutamate-induced nociception, both treatments reduced pain behavior, but only PHE-HPβCD decreased eye wipes in corneal nociception. These results suggest that PHE, especially in complexed form, alleviates orofacial pain by potentially modulating pain-related receptors (TRPA1 and TRPV1), mediators, like glutamate, and reducing pro-inflammatory cytokines. Further research is needed to explore the precise mechanisms of PHE in chronic orofacial pain models, but the study indicates promising avenues for new pain treatments.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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