Development and Application of an Indirect Enzyme-Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs.

Autor: Castañeda Montes FJ; Estancias Posdoctorales por México para la Formación y Consolidación de las y los Investigadores por México, CONAHCYT, Mexico City, Mexico.; Posgrado en Ciencias de la Producción y de la Salud Animal, Facultad de Estudios Superiores, Universidad Nacional Autónoma de México, Cuautitlan, Estado de México, Mexico., Cerriteño Sánchez JL; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Mexico City, Mexico., Cuevas-Romero JS; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Mexico City, Mexico., Castañeda Montes MA; Dirección de Procesos Alimentarios y Química Área Biotecnología, Unidad Académica de Capulhuac, Universidad Tecnológica del Valle de Toluca, Lerma, Mexico.; Laboratorio de Virología, Genética y Biología Molecular, Facultad de Educación Superior, Cuautitlán, Medicina Veterinaria, Universidad Nacional Autónoma de México, Cuautitlán Izcalli, Estado de México, Mexico., Mendoza Elvira S; Posgrado en Ciencias de la Producción y de la Salud Animal, Facultad de Estudios Superiores, Universidad Nacional Autónoma de México, Cuautitlan, Estado de México, Mexico.
Jazyk: angličtina
Zdroj: Veterinary medicine and science [Vet Med Sci] 2024 Nov; Vol. 10 (6), pp. e70108.
DOI: 10.1002/vms3.70108
Abstrakt: Porcine deltacoronavirus (PDCoV) is an infectious disease that causes diarrhoea in pigs of different ages; however, piglets are more susceptible. PDCoV was first reported in 2012 in China and Hong Kong. Later, it was first reported in the USA in 2014 and in Mexico in 2019. Several studies have shown that M protein is highly conserved and, therefore, suitable for diagnostic systems. In this study, for the first time, an indirect enzyme-linked immunosorbent assay (iELISA) based on a recombinant M protein (rM-PDCoV) was developed to evaluate the seroprevalence of PDCoV in four states in Mexico. High sensitivity (83%) and specificity (100%) were observed for the iELISA. The kappa index calculated a nearly perfect agreement (0.8831) compared to the Western blot (gold standard test), suggesting acceptable statistical value support. In this study, 50.38% of the serum samples from backyard pigs were PDCoV-positive. The serological comparison showed that PDCoV/PEDV coinfections occurred in 31.98% of the analysed sera. These results can enrich our understanding of how this virus spreads and enable the evaluation of PDCoV infections. Moreover, it highlights the importance of continually investigating the seroprevalence of PDCoV in Mexico because there is also no information about the current prevalence of the disease.
(© 2024 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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