Installation of an organocatalyst into a protein scaffold creates an artificial Stetterase.

Autor: MacAulay A; School of Chemistry, University of Edinburgh, Joseph Black Building, King's Buildings, Edinburgh, EH9 3FJ, UK. Dominic.Campopiano@ed.ac.uk., Klemencic E; School of Chemistry, University of Edinburgh, Joseph Black Building, King's Buildings, Edinburgh, EH9 3FJ, UK. Dominic.Campopiano@ed.ac.uk., Brewster RC; School of Chemistry, University of Edinburgh, Joseph Black Building, King's Buildings, Edinburgh, EH9 3FJ, UK. Dominic.Campopiano@ed.ac.uk., Ünal SM; School of Biological Sciences, University of Edinburgh, Roger Land Building, King's Buildings, Edinburgh, EH9 3FF, UK., Notari E; School of Chemistry, University of Edinburgh, Joseph Black Building, King's Buildings, Edinburgh, EH9 3FJ, UK. Dominic.Campopiano@ed.ac.uk.; School of Biological Sciences, University of Edinburgh, Roger Land Building, King's Buildings, Edinburgh, EH9 3FF, UK., Wood CW; School of Biological Sciences, University of Edinburgh, Roger Land Building, King's Buildings, Edinburgh, EH9 3FF, UK., Jarvis AG; School of Chemistry, University of Edinburgh, Joseph Black Building, King's Buildings, Edinburgh, EH9 3FJ, UK. Dominic.Campopiano@ed.ac.uk., Campopiano DJ; School of Chemistry, University of Edinburgh, Joseph Black Building, King's Buildings, Edinburgh, EH9 3FJ, UK. Dominic.Campopiano@ed.ac.uk.
Jazyk: angličtina
Zdroj: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2024 Nov 19; Vol. 60 (93), pp. 13746-13749. Date of Electronic Publication: 2024 Nov 19.
DOI: 10.1039/d4cc05182c
Abstrakt: Using a protein scaffold covalently functionalised with a thiamine-inspired N-heterocyclic carbene (NHC), we created an artificial Stetterase (ArtiSt) which catalyses a stereoselective, intramolecular Stetter reaction. We demonstrate that ArtiSt functions under ambient conditions with low catalyst loading. Furthermore, activity can be increased >20 fold by altering the protein scaffold.
Databáze: MEDLINE
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