Bactericidal and Synergistic Effects of Lippia origanoides Essential Oil and Its Main Constituents against Multidrug-Resistant Strains of Acinetobacter baumannii .

Autor:	da Silva AT; Animal Microbiology and Immunology Laboratory, Universidade Federal do Vale do São Francisco (UNIVASF), Campus Agricultural Sciences, Petrolina, Pernambuco 56300-000, Brazil., Cândido AECM; Animal Microbiology and Immunology Laboratory, Universidade Federal do Vale do São Francisco (UNIVASF), Campus Agricultural Sciences, Petrolina, Pernambuco 56300-000, Brazil., Júnior EDCM; Animal Microbiology and Immunology Laboratory, Universidade Federal do Vale do São Francisco (UNIVASF), Campus Agricultural Sciences, Petrolina, Pernambuco 56300-000, Brazil., do É GN; Animal Microbiology and Immunology Laboratory, Universidade Federal do Vale do São Francisco (UNIVASF), Campus Agricultural Sciences, Petrolina, Pernambuco 56300-000, Brazil., Moura MPS; College of Pharmaceutical Sciences (CFARM), Universidade Federal do Vale do São Francisco (UNIVASF), Av. José de Sá Maniçoba, Centro, Petrolina, Pernambuco 56304-205, Brazil., Souza RFS; Animal Microbiology and Immunology Laboratory, Universidade Federal do Vale do São Francisco (UNIVASF), Campus Agricultural Sciences, Petrolina, Pernambuco 56300-000, Brazil., Guimarães ML; Laboratory of Impedance Spectroscopy and Organic Materials, Institute of Materials Science, Universidade Federal do Vale do São Francisco (UNIVASF), Juazeiro, Bahia 48902-300, Brazil., Peixoto RM; Animal Microbiology and Immunology Laboratory, Universidade Federal do Vale do São Francisco (UNIVASF), Campus Agricultural Sciences, Petrolina, Pernambuco 56300-000, Brazil., de Oliveira HP; Laboratory of Impedance Spectroscopy and Organic Materials, Institute of Materials Science, Universidade Federal do Vale do São Francisco (UNIVASF), Juazeiro, Bahia 48902-300, Brazil., da Costa MM; Animal Microbiology and Immunology Laboratory, Universidade Federal do Vale do São Francisco (UNIVASF), Campus Agricultural Sciences, Petrolina, Pernambuco 56300-000, Brazil.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2024 Oct 16; Vol. 9 (43), pp. 43927-43939. Date of Electronic Publication: 2024 Oct 16 (Print Publication: 2024).
DOI:	10.1021/acsomega.4c07565
Abstrakt:	Bacterial resistance in Acinetobacter baumannii is a significant public health challenge, as these bacteria can evade multiple antibiotics, leading to difficult-to-treat infections with high mortality rates. As part of the search for alternatives, essential oils from medicinal plants have shown promising antibacterial potential due to their diverse chemical constituents. This study evaluated the antibacterial, antibiofilm, and synergistic activities of the essential oil of Lippia origanoides (EOLo) and its main constituents against multidrug-resistant clinical isolates of A. baumannii . Additionally, the antibacterial and antibiofilm potential of a nanoemulsion containing carvacrol (NE-CAR) was assessed. EOLo was extracted through hydrodistillation, and its components were identified via gas chromatography coupled with mass spectrometry. The A. baumannii isolates ( n = 9) were identified and tested for antimicrobial susceptibility using standard disk diffusion methods. Antibacterial activity was determined by broth microdilution, while antibiofilm activity was measured using colorimetric methods with crystal violet and scanning electron microscopy. Synergism tests with antibiotics (meropenem, ciprofloxacin, gentamicin, and ampicillin+sulbactam) were performed using the checkerboard method. The primary constituents of EOLo included carvacrol (48.44%), p -cymene (14.58%), and thymol (10.16%). EOLo, carvacrol, and thymol demonstrated significant antibacterial activity, with carvacrol showing the strongest effect. They were also effective in reducing biofilm formation, as was NE-CAR. The combinations with antibiotics revealed significant synergistic effects, lowering the minimum inhibitory concentration of the tested antibiotics. Therefore, this study confirms the notable antibacterial activity of the essential oil of L. origanoides and its constituents, especially carvacrol, suggesting its potential as a therapeutic alternative for A. baumannii infections. Competing Interests: The authors declare no competing financial interest. (© 2024 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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