Antimalarial efficacy of Duttaphrynus melanostictus skin extract via inhibition of Plasmodium falciparum Na + /H + ATPase.

Autor: Bagwe AD; Department of Zoology, Sophia College for Women (Autonomous), Mumbai, Maharashtra 400 026 India.; Department of Zoology, KET's V. G. Vaze College of Arts, Science and Commerce (Autonomous), Mumbai, Maharashtra 400 081 India., D'Souza RC; Department of Zoology, Sophia College for Women (Autonomous), Mumbai, Maharashtra 400 026 India., Sharma BB; Department of Zoology, KET's V. G. Vaze College of Arts, Science and Commerce (Autonomous), Mumbai, Maharashtra 400 081 India.
Jazyk: angličtina
Zdroj: Journal of parasitic diseases : official organ of the Indian Society for Parasitology [J Parasit Dis] 2024 Dec; Vol. 48 (4), pp. 831-848. Date of Electronic Publication: 2024 Aug 02.
DOI: 10.1007/s12639-024-01716-9
Abstrakt: Malaria remains a major health issue worldwide that affects many people, particularly in developing nations. Since, the malarial parasite has developed resistance against nearly every antimalarial drug now in use, it is imperative to search for novel antimalarial medications. Toxins produced by skin glands of toads have been shown to possess antiparasitic properties against a variety of protozoan parasites because of the bufadienolides they contain. Even though several studies have been conducted to show that toad skin secretions have antimalarial properties, very little information is known about the precise mechanism by which they work against Plasmodium infection. Thus, the goal of this study was to evaluate the antiplasmodial activity of crude skin extracts from Common Asian Toads, Duttaphrynus melanostictus , of different sizes and illustrate how they work against Plasmodium falciparum 3D7 cells. The findings demonstrated a negative correlation between the toad size and percent yield of the extracts. HPTLC and UPLC-MS/MS analysis of the extracts exhibited varied composition of bufadienolides depending on the size of the animal. The extract obtained from small toads containing resibufagin and marinobufagin lactate demonstrated highest antiplasmodial activity and showed lowest cytotoxicity on peripheral blood mononuclear cells. It was discovered that the extract was effective against the trophozoite stage of the parasite. The extract was reported to inhibit Na + /H + ATPase of Plasmodium by binding to sodium-enzyme complex at ATP binding site. The study offers baseline data that can be used to assess the antimalarial potential of individual components in the skin extract derived from small toads.
Supplementary Information: The online version contains supplementary material available at 10.1007/s12639-024-01716-9.
Competing Interests: Conflict of interestThe authors have no conflict of interest to declare.
(© Indian Society for Parasitology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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