The effect of titanium surface treatment by application of constant potential or current on the viability of pre-osteoblast cells: an in-vitro study.

Autor:	Cai W; Faculty of Dental Medicine and Oral Health Sciences, McGill University, Montreal, QC, Canada., Wang M; State Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, Wuhan, China.; Department of Oral Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, China., Hadad AE; Faculty of Dental Medicine and Oral Health Sciences, McGill University, Montreal, QC, Canada., Zhang Y; Faculty of Dental Medicine and Oral Health Sciences, McGill University, Montreal, QC, Canada., Tran SD; Faculty of Dental Medicine and Oral Health Sciences, McGill University, Montreal, QC, Canada., Shurbaji S; College of Dental Medicine, QU Health, Qatar University, Doha, Qatar., Nasrallah GK; Department of Biomedical Science, College of Health Sciences, QU Health, Qatar University, Doha, Qatar., Sanz M; ETEP Research Group, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain., Omanovic S; Chemical Engineering department, McGill University, Montreal, QC, Canada., Tamimi F; College of Dental Medicine, QU Health, Qatar University, Doha, Qatar.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2024 Oct 18; Vol. 12, pp. 1425450. Date of Electronic Publication: 2024 Oct 18 (Print Publication: 2024).
DOI:	10.3389/fbioe.2024.1425450
Abstrakt:	Objectives: The aim of this study was to investigate the impact of electrochemical treatment of a titanium surface employing constant current and potential on the viability of the tissue cells attached to the surface and determining the safety limits for this type of treatment. Methods: Pre-osteoblast cells (pOB) were cultured and seeded onto titanium discs. The cell-seeded discs were then exposed to a range of contant direct electrical potentials (-6V-6V) or contant direct electrical currents (-12.5 mA, -25 mA, or -50 mA) using a three-electrode system connected to a potentiostat. Cell viability was assessed using live/dead assay and fluorescence microscopy. Results: Exposure of cells to high negative potentials caused cell detachment, while exposure to positive ones led to cell death on the cpTi surfaces. However, cellular viability was preserved when the electrical potentials were kept between -3 and +3 V. Cells retained 80% viability when subjected to -12.5 mA currents with an initial pOB cell count of 5 × 10 4 . However, when the initial cell count was elevated to 1 × 10 5 , the cells demonstrated the ability to withstand an even greater current (-25 mA) while preserving their vitality at the same level. Conclusion: Treatment of a titanium dental implant surface employing constant potential or current can harm cells surrounding dental implants. However, this damage can be minimized by keeping the potential within a safety limit. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Cai, Wang, Hadad, Zhang, Tran, Shurbaji, Nasrallah, Sanz, Omanovic and Tamimi.)
Databáze:	MEDLINE
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