Elucidating the anticancerous efficacy of genistein via modulating HPV (E7 and E6) oncogenes expression and apoptotic induction in cervical cancer cells.

Autor: Pandey P; Centre for Research Impact & Outcome, Chitkara University Institute of Engineering & Technology, Chitkara University, Rajpura, Punjab, India.; Chitkara Centre for Research and Development, Chitkara University, Baddi, Himachal Pradesh, India., Ramniwas S; University Centre of Research and Development, University institute of Biotechnology, Chandigarh University, Gharuan, Mohali, Punjab, India., Pandey S; School of Applied and Life Sciences, Uttaranchal University, Dehradun, Uttarakhand, India., Lakhanpal S; School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India., Ballal S; Department of Chemistry and Biochemistry, School of Sciences, JAIN (Deemed to be University), Bangalore, Karnataka, India., Kumar S; Department of Allied Healthcare and Sciences, Vivekananda Global University, Jaipur, Rajasthan, India., Bhat M; Department of Medicine, National Institute of Medical Sciences, NIMS University Rajasthan, Jaipur, Rajasthan, India., Sharma S; Chandigarh Pharmacy College, Chandigarh Group of Colleges-Jhanjeri, Mohali, Punjab, India., Kumar MR; Department of Chemistry, Raghu Engineering College, Visakhapatnam, Andhra Pradesh, India., Khan F; Center for Global Health Research Saveetha Medical College, Saveetha Institute of Medical and Technical Sciences, Chennai, India.
Jazyk: angličtina
Zdroj: Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2024 Nov 03. Date of Electronic Publication: 2024 Nov 03.
DOI: 10.1002/bab.2691
Abstrakt: In recent years, genistein has garnered increased interest for its ability to inhibit numerous deregulated targets associated with cancer progression and induction of programmed cell death and antiproliferative activities in human carcinoma cells. Cancer etiology is influenced via multiple disrupted signaling pathways. This study therefore directed toward investigating genistein efficacy in modulating mRNA expression levels of two crucial Human Pappiloma Virus (HPV) (E7 and E6) oncogenes for cancer treatment. Moreover, the inhibitory effects of genistein for HPV (E7 and E6) oncogenes in cervical carcinoma have not yet been reported. Current study investigated inhibitory potential of genistein in HPV (E7 and E6) oncogenes in HeLa cells. These oncogenes are known to deactivate many tumor suppressor proteins (p53 and pRB). Genistein therapy resulted in decreased cell proliferation and increased cell accumulation in the G (G0/G1) phase in HeLa cell lines. In addition, genistein therapy has resulted in the suppression of HPV (E7 and E6) gene expression and simultaneously increasing expression levels of p53 and pRB mRNA levels. As a consequence, there has been an activation of a series of caspases (3, 8, and 9), resulting in their cleavage. Consequently, our data suggests that genistein could be a powerful candidate for treating cervical cancer by targeting two important oncogenes involved in viral development. However, more in vitro research on primary cervical cancer cells is required to validate the clinically relevant efficacy of genistein against cervical cancer.
(© 2024 International Union of Biochemistry and Molecular Biology, Inc.)
Databáze: MEDLINE
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