Characterization and functional analysis of pl-miR-2188 in melanin synthesis in leopard coral grouper (Plectropomus leopardus).

Autor: Hao R; Development and research center for biological marine resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China. Electronic address: haorj@zjblab.com., Li L; Development and research center for biological marine resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China; Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China., Zhang D; Development and research center for biological marine resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China., Tian Y; Development and research center for biological marine resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China; Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China., Long H; Development and research center for biological marine resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China; Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China., Li H; Development and research center for biological marine resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China., Zhu X; Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang 524088, China., Huang Y; Development and research center for biological marine resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China; Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China., Li G; Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang 524088, China., Zhu C; Development and research center for biological marine resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524006, China; Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang 524088, China. Electronic address: chz416@163.com.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2025 Jan; Vol. 275, pp. 111043. Date of Electronic Publication: 2024 Nov 02.
DOI: 10.1016/j.cbpb.2024.111043
Abstrakt: MicroRNAs (miRNAs) are known to regulate gene expression and play a role in body color formation in fish. However, the molecular mechanisms underlying miRNA involvement in the body color of leopard coral grouper (Plectropomus leopardus) remain largely unexplored. In this study, we investigated the expression levels of miR-2188 in red and black P. leopardus (pl-miR-2188) and found significantly higher expression levels in red fish samples compared to those in black fish samples. Silencing pl-miR-2188 in vivo using a pl-miR-2188 antagomir resulted in increased melanin concentration. Following pl-miR-2188 silencing, the expression levels of melanin-related genes, such as tyrosinase (tyr), TYR-related protein 1 (tyrp1-1 and tyrp1-2) and TYR-related protein 2 (tyrp2), and microphthalmia-associated transcription factor (mitf), were elevated. RNAhybrid predictions and dual-luciferase reporter assays identified sox5 as a target mRNA of pl-miR-2188. Following pl-miR-2188 antagomir injection, sox5 expression was significantly upregulated in the injection group compared to that in control groups (P < 0.05). These results suggest that pl-miR-2188 may regulate melanin synthesis in P. leopardus by targeting sox5. This study provides new insights into the miRNA-mRNA interactions involved in melanin synthesis and body color formation in the leopard coral grouper.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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