Characterization and phylogenetic analysis of the complete mitochondrial genome of Rhabdias kafunata (Rhabditida: Rhabdiasidae).

Autor: Li YX; College of Animal Sciences (College of Bee Science) Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Laboratory of Animal Medical Engineering of Fujian Province, Fuzhou, 350002, China., Huang XH; College of Animal Sciences (College of Bee Science) Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Laboratory of Animal Medical Engineering of Fujian Province, Fuzhou, 350002, China., Li MR; College of Animal Sciences (College of Bee Science) Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Laboratory of Animal Medical Engineering of Fujian Province, Fuzhou, 350002, China., Li SY; College of Animal Sciences (College of Bee Science) Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Laboratory of Animal Medical Engineering of Fujian Province, Fuzhou, 350002, China., Huang ZJ; College of Animal Sciences (College of Bee Science) Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Laboratory of Animal Medical Engineering of Fujian Province, Fuzhou, 350002, China., Wang DF; College of Animal Sciences (College of Bee Science) Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Laboratory of Animal Medical Engineering of Fujian Province, Fuzhou, 350002, China., Yin GW; College of Animal Sciences (College of Bee Science) Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Laboratory of Animal Medical Engineering of Fujian Province, Fuzhou, 350002, China. Electronic address: yinguangwen000@sina.com., Wang L; College of Animal Sciences (College of Bee Science) Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Laboratory of Animal Medical Engineering of Fujian Province, Fuzhou, 350002, China. Electronic address: dkwangl@fafu.edu.cn.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2023 Oct 27, pp. 108646. Date of Electronic Publication: 2023 Oct 27.
DOI: 10.1016/j.exppara.2023.108646
Abstrakt: Rhabdias kafunata (Rhabditida: Rhabdiasidae) is a parasitic nematode that significantly affects bufonids. To better understand the genome-level characteristics of related species, Illumina sequencing was used to identify mitochondrial genes and analyze their basic characteristics and gene arrangements. The mitogenome of R. kafunata is 14,068 bp in length and contains 36 genes, including 12 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes and one noncoding region (NCR). The nucleotide composition is highly biased toward A + T, accounting for 75.5% of the entire mitochondrial genome. The cox1 sequence is relatively conserved in Ka/Ks analyses and can be used as a gene fragment for species identification. While 8 of the 12 PCGs use the typical ATN initiation codon, nad1-2, nad4, and cox3 utilize a TTG initiation codon. Most stop codons end with the standard TAA or TAG, except for cytb, which ends with an incomplete TA. Additionally, trnM, trnK, and trnI have the typical clover-leaf secondary structure, while the remaining tRNAs lack the DHU arm or TΨC arm. Phylogenetic analysis indicates that R. kafunata belongs to the Rhabditidae family and is closely related to Litoditis marina and Caenorhabditis angaria among sequenced lepidopteran mitochondrial genomes.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personalrelationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier Inc.)
Databáze: MEDLINE
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