A comprehensive study on mechanisms of action of fibroin, aloe vera, and ginger extracts through histochemical, inflammation biomarkers, and matrix metalloproteinases analysis against diabetic wounds.

Autor: Sulayman R; Medical Toxicology and Biochemistry Laboratory, Department of Zoology, Government College University, Lahore, Pakistan., Ali S; Medical Toxicology and Biochemistry Laboratory, Department of Zoology, Government College University, Lahore, Pakistan. Electronic address: dr.shaukatali@gcu.edu.pk., Ullah R; Department of Zoology, Mirpur University of Science and Technology (MUST), Mirpur, 10250, AJK, Pakistan., Summer M; Medical Toxicology and Biochemistry Laboratory, Department of Zoology, Government College University, Lahore, Pakistan., Hassan A; Medical Toxicology and Biochemistry Laboratory, Department of Zoology, Government College University, Lahore, Pakistan., Shahzad H; Medical Toxicology and Biochemistry Laboratory, Department of Zoology, Government College University, Lahore, Pakistan., Fiaz U; Medical Toxicology and Biochemistry Laboratory, Department of Zoology, Government College University, Lahore, Pakistan., Mumtaz S; Medical Toxicology and Biochemistry Laboratory, Department of Zoology, Government College University, Lahore, Pakistan., Nauroze T; Department of Zoology, University of Education, Lahore, Pakistan., Mumtaz S; Department of Zoology, University of Poonch, Rawalakot, AJK, Pakistan.
Jazyk: angličtina
Zdroj: Journal of tissue viability [J Tissue Viability] 2024 Nov; Vol. 33 (4), pp. 949-959. Date of Electronic Publication: 2024 Oct 21.
DOI: 10.1016/j.jtv.2024.10.006
Abstrakt: Introduction: Diabetes causes complications like delayed wound healing for a long time. Fibroin, aloe vera, and ginger extracts along with their combinations are used for diabetic wound healing.
Methods: After induction of diabetes, The wound healing effects of fibroin (50 mg/ml), aloe vera gel (50 mg/ml), and ginger extract (30 mg/ml), individually and in combination, were assessed. The pro-inflammatory cytokines including tumor narcosis factor-α (TNF-α) interleukin (IL-6, IL-8, IL-1β), matrix metalloproteinases (MMP 2, MMP7, MMP 9), vascular endothelial growth factor (VEGF) and tissue inhibitors of metalloproteinases (TIMPs) levels were analyzed in the serum.
Results: A combination of fibroin + aloe vera gel + ginger extract (Fi + Al + Gi) healed the wounds in 11 days via wound contraction of 98.5 ± 0.9 % as compared to diabetic control (58.2 ± 0.7 %) and positive control (73.3 ± 0.6 %) groups. However, the wounds of the Polyfax and the diabetic control groups were healed in 17 and 19 days, corresponding to a contraction of: 96.7 ± 1.4 % and 96.3 ± 1.1 %. The histological assay showed that the Fi + Al + Gi group indicated an increased growth of collagen fibers, fibroblasts, keratinocytes and blood vessels with lessened inflammation. The Fi + Al + Gi group alleviated the serum level of TNF-α (12.7 ± 0.9 pg/ml), IL-6 (9.6 ± 0.9 pg/ml), IL-8 (19.6 ± 1.0 pg/ml), MMP2 (217.0 ± 9.2 pg/ml), MMP7 (279.0 ± 9.8 pg/ml), and MMP9 (156.0 ± 11.6 pg/ml) significantly as compared to the diabetic control (P ≤ 0.05). TIMP serum level (202.0 ± 6.9 pg/ml) was significantly elevated as compared to the diabetes control group.
Conclusion: The present study concludes that the biomaterials in their combinations possess high regenerative and healing abilities.
Competing Interests: Declaration of competing interest Authors declare no conflict of interest.
(Copyright © 2024 Tissue Viability Society / Society of Tissue Viability. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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