Kefir peptides mitigate L-NAME-induced preeclampsia in rats through modulating hypertension and endothelial dysfunction.

Autor: Chen YH; Department of Life Sciences, Doctoral Program in Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan. Electronic address: yhchen1218@smail.nchu.edu.tw., Chang YC; Department of Life Sciences, Doctoral Program in Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan. Electronic address: sandybiology2018@gmail.com., Wu WJ; Department of Life Sciences, Doctoral Program in Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan; Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua 50006, Taiwan. Electronic address: crystalwu835@gmail.com., Chen M; Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua 50006, Taiwan; Department of Genomic Medicine, Changhua Christian Hospital, Changhua 50046, Taiwan. Electronic address: mingchenmd@gmail.com., Yen CC; Department of Internal Medicine, China Medical University Hospital, China Medical University, Taichung 404, Taiwan. Electronic address: d5210@mail.cmuh.org.tw., Lan YW; Department of Life Sciences, Doctoral Program in Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan; Phoenix Children's Health Research Institute, Department of Child Health, University of Arizona College of Medicine, Phoenix 85004, USA. Electronic address: yingweilan@arizona.edu., Cheng HC; Department of Life Sciences, Doctoral Program in Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan; The iEGG and Animal Biotechnology Center, and Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan. Electronic address: hcheng@dragon.nchu.edu.tw., Chen CM; Department of Life Sciences, Doctoral Program in Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan; The iEGG and Animal Biotechnology Center, and Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan; Center for General Educational, National Quemoy University, Kinmen 892, Taiwan. Electronic address: chchen1@dragon.nchu.edu.tw.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2024 Nov; Vol. 180, pp. 117592. Date of Electronic Publication: 2024 Oct 28.
DOI: 10.1016/j.biopha.2024.117592
Abstrakt: Aim: Preeclampsia is a complex and serious pregnancy disorder that leads to maternal and neonatal mortality worldwide. Kefir peptides (KPs), derived from various prebiotic fermentations in whole milk by kefir grains, were investigated for their potential therapeutic effects. In this study, we used the L-NAME in drinking water to induce a preeclampsia-like condition in spontaneous hypertension stroke-prone (SHRSP) pregnant rats.
Main Methods: The rats were assigned to five groups: the normal group (WKY rats), the untreated group (SHRSP rat control pregnant), the L-NAME/Mock group (SHRSP rats fed with L-NAME water), the L-NAME/KPs-LD group (SHRSP rats fed with L-NAME water and low-dose KPs diets), and the L-NAME/KPs-HD group (SHRSP rats fed with L-NAME water and high-dose KPs diets) for a 20-day experiment. Chorioallantois membrane (CAM) assay was applied for ex vivo angiogenesis study of KPs treatment.
Key Findings: Data showed that rats in the L-NAME group developed severe hypertension, proteinuria, placental damage, and embryo resorption. Pre-administration of KPs significantly reduced hypertension, proteinuria, improved generalized endothelial dysfunction, and decreased levels of anti-HIF-1α, sFLT1, anti-TNF-α, and IL-6 in the placenta of SHRSP rats. In ex vivo CAM study, L-NAME administration in chicken embryos resulted in lower vessel density and hemorrhage; however, angiogenesis was observed after KPs-HD treatment.
Significance: The results indicate that kefir peptides improve renal lesions, prevent renal parenchyma damage, and balance endothelial and angiogenic dysfunction in both maternal and fetal sites in L-NAME-induced SHRSP pregnant rats.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Chuan-Mu Chen reports financial support was provided by Ministry of Science and Technology in Taiwan. Chuan-Mu Chen reports financial support was provided by Ministry of Education in Taiwan. There are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
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