Osteoblasts win the race for the surface on DNA polyelectrolyte multilayer coatings against S. epidermidis but not against S. aureus.

Autor: Covato C; Department of Bionanosciences, Institute of Colloid and Biointerface Science, BOKU University, Muthgasse 11, Vienna 1190, Austria., Pilipenco A; FZU - Institute of Physics of the Czech Academy of Sciences, Na Slovance 1999/2, Prague 18200, Czech Republic., Scheberl A; Department of Bionanosciences, Institute of Colloid and Biointerface Science, BOKU University, Muthgasse 11, Vienna 1190, Austria., Reimhult E; Department of Bionanosciences, Institute of Colloid and Biointerface Science, BOKU University, Muthgasse 11, Vienna 1190, Austria., Subbiahdoss G; Department of Bionanosciences, Institute of Colloid and Biointerface Science, BOKU University, Muthgasse 11, Vienna 1190, Austria. Electronic address: guruprakash.subbiahdoss@boku.ac.at.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2025 Jan; Vol. 245, pp. 114336. Date of Electronic Publication: 2024 Oct 24.
DOI: 10.1016/j.colsurfb.2024.114336
Abstrakt: Biomaterial-associated infections pose severe challenges in modern medicine. Previously, we reported that polyanionic DNA surface coatings repel bacterial adhesion and support osteoblast-like cell attachment in monoculture experiments, candidate for orthopaedic implant coatings. However, monocultures lack the influence of bacteria or bacterial toxins on osteoblast-like cell adhesion to biomaterial surfaces. In this study, co-culture of staphylococcus (S. epidermidis and S. aureus) and SaOS-2 osteosarcoma cells was studied on chitosan-DNA polyelectrolyte multilayer coated glass based on the concept of `the race for the surface`. Staphylococcus was first deposited onto the surface in a microfluidic chamber to mimic peri-operative contamination, and subsequently, SaOS-2 cells were seeded. Both staphylococcus and SaOS-2 cells were cultured together on the surfaces for 24 h under flow. The presence of S. epidermidis decreased SaOS-2 cell number on all surfaces after 24 h. However, the cells that adhered spread equally well in the presence of low virulent S. epidermidis. However, highly virulent S. aureus induced cell death of all adherent SaOS-2 cells on chitosan-DNA multilayer coated glass, a worse outcome than on uncoated glass. The outcome of our co-culture study highlights the limitations of monoculture models. It demonstrates the need for in vitro co-culture assays to meaningfully bridge the gap in lab testing of biomaterials and their clinical evaluations where bacterial infection can occur. The relative failure of cell-adhesive and bacteria-repelling DNA coatings in co-cultures also suggests the need to incorporate bactericidal in addition to non-adhesive functions to protect competitive cell spreading over a long period.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Guruprakash Subbiahdoss reports financial support was provided by Hochschuljubiläumsfonds, City of Vienna. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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