A liquid crystal-decorated aptasensing gadget for rapid monitoring of A549 cells: Future portable test kit for lung cancer diagnosis.

Autor: Khoshbin Z; Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran., Mohammadi F; Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran., Naderpour K; Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran., Ramezani M; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran., Alibolandi M; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran., Abnous K; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: abnouskh@mums.ac.ir., Taghdisi SM; Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: taghdisihm@mums.ac.ir.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2024 Nov 22; Vol. 1330, pp. 343276. Date of Electronic Publication: 2024 Sep 24.
DOI: 10.1016/j.aca.2024.343276
Abstrakt: Background: Presented here is a straightforward detection system designed to track non-small cell lung cancer (specifically A549 cells) using a combination of liquid crystals (LCs) and aptamer sequences, marking a pioneering approach in this field. A change in the alignment of LCs from perpendicular to random status by the aptamer-cell complex altered the murky polarized background of the aptasensor to multicolored.
Results: The LC-designed aptasensor could determine A549 cancerous cells in the range of 2.0E+01-7.0E+07 cell mL -1 with a limit of detection (LOD) as low as 10 cell mL -1 . Through precise quantification of A549 cells in human serum samples diluted 20 times, with recovery rates ranging from 97.59 % to 101.31 %, the suggested aptasensor proves to be a dependable method for cancer screening. Furthermore, the LC aptasensor was identified as a fast sensing array due to a 10-min incubation period for the aptamer-cell complexation.
Significance: The LC aptasensor is label-free, operator-independent, low-cost, sensitive, and user-friendly, making it potent as a miniaturized portable sensing chip for efficient healthcare monitoring.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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