Influence of bisphenol A and its analogues on human gut microbiota composition and metabolic activity: Insights from an in vitro model.

Autor: Średnicka P; Laboratory of Biotechnology and Molecular Engineering, Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology - State Research Institute, Rakowiecka 36 Street, Warsaw, Poland. Electronic address: paulina.srednicka@ibprs.pl., Roszko M; Department of Food Safety and Chemical Analysis, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology - State Research Institute, Rakowiecka 36 Street, Warsaw, Poland., Emanowicz P; Laboratory of Biotechnology and Molecular Engineering, Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology - State Research Institute, Rakowiecka 36 Street, Warsaw, Poland., Wójcicki M; Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology - State Research Institute, Rakowiecka 36 Street, Warsaw, Poland., Popowski D; Department of Food Safety and Chemical Analysis, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology - State Research Institute, Rakowiecka 36 Street, Warsaw, Poland; Natural Products & Food Research and Analysis - Pharmaceutical Technology, Faculty of Pharmacy, University of Antwerp, Universiteitplein 1, Wilrijk, Belgium., Kanabus J; Department of Food Safety and Chemical Analysis, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology - State Research Institute, Rakowiecka 36 Street, Warsaw, Poland., Juszczuk-Kubiak E; Laboratory of Biotechnology and Molecular Engineering, Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology - State Research Institute, Rakowiecka 36 Street, Warsaw, Poland.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Dec 15; Vol. 956, pp. 177323. Date of Electronic Publication: 2024 Nov 06.
DOI: 10.1016/j.scitotenv.2024.177323
Abstrakt: Food contamination is a primary route of human exposure to bisphenols (BPs), which are known to affect gut microbiota (GM) and intestinal health. This study comprehensively assessed the impact of bisphenol A (BPA) and three of its substitutes-bisphenol S (BPS), bisphenol F (BPF), and tetramethyl bisphenol F (TMBPF, the monomer of valPure V70) - on the taxonomic and functional profile of human GM using an in vitro model. Human GM was acutely exposed to 1 mM concentrations of these BPs during a 48 h anaerobic cultivation. We first examined the effects of BPA, BPS, BPF, and TMBPF on GM taxonomic and metabolic profiles, mainly focusing on short-chain fatty acids (SCFAs) production. We then evaluated the degradation potential of these BPs by GM and its influence on their estrogenic activity. Finally, we assessed the impact of GM metabolites from BPs-exposed cultures on the viability of intestinal epithelial cells (Caco-2). BPA, BPS, and BPF severely disrupted GM taxonomic composition and metabolite profiles, significantly reducing SCFAs production. In contrast, TMBPF exhibited the least disruptive effects, suggesting it may be a safer alternative. Although the GM did not biotransform the BPs, bioadsorption occurred, with affinity correlating to hydrophobicity in the order of TMBPF > BPA > BPF > BPS. GM reduced the estrogenic activity of BPs primarily through bioadsorption. However, exposure of gut epithelial cells to Post-Culture Supernatants of BPA, BPF, and TMBPF significantly reduced Caco-2 cell viability, indicating the potential formation of harmful GM-derived metabolites and/or a depletion of beneficial GM metabolites.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this manuscript.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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