Antibacterial peptides from black soldier fly (Hermetia illucens) larvae: mode of action and characterization.

Autor: Pimchan T; School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand., Hamzeh A; School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand., Siringan P; School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand., Thumanu K; Synchrotron Light Research Institute, Nakhon Ratchasima, 30000, Thailand., Hanboonsong Y; Department of Entomology, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand., Yongsawatdigul J; School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand. jirawat@sut.ac.th.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Nov 02; Vol. 14 (1), pp. 26469. Date of Electronic Publication: 2024 Nov 02.
DOI: 10.1038/s41598-024-73766-1
Abstrakt: Antibacterial peptides from black soldier fly larvae extract were prepared using Flash column chromatography. Three out of five fractions (F 2 , F 3 and F 4 ) showed antibacterial activity against Listeria monocytogenes DMST 17303 with a minimum inhibitory concentration (MIC) of 1 mM, followed by Salmonella enterica Enteritidis DMST 15679 and Escherichia coli O157:H7 DMST 12743 with a MIC ranging from 4 to 8 mM. Due to the higher yield, F 2 and F 3 were further analyzed on their mode of action against L. monocytogenes DMST 17303. Both fractions, particularly F 2 , exerted antibacterial activity through inducing bacterial cell membrane disintegration and interaction with intracellular compounds including fatty acids, proteins, and nucleic acids. The F 3 did not show significant hemolytic activity up to 4 mM, while F 2 showed lower than 5% hemolysis up to 8 mM. Time-to-kill analysis revealed that F 2 was more effective and exerted a sustainable killing effect after 2 and 4 h, depending on the concentration of 1 and 2×MIC, respectively, while the F 3 at 2×MIC could completely kill the test bacteria within 24 h. Among the identified peptides in the fractions, those with charged, either positively or negatively, and moderate hydrophobicity of ranging 6.68-15.70, namely CGPPRQGPFPR, HLEEELK, LEEAEERAD, TEELEEAKKK, and KGNSELEEAKKK, are potential antimicrobial peptides.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: