Machine learning assisted dual-modal SERS detection for circulating tumor cells.

Autor:	Zhang C; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China., Xu L; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China., Miao X; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China., Zhang D; Zhejiang Cancer Hospital, Hangzhou, 310022, PR China., Xie Y; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China. Electronic address: xieyujiao@nimte.ac.cn., Hu Y; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China., Zhang Z; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China., Wang X; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China., Wu X; Zhejiang Cancer Hospital, Hangzhou, 310022, PR China., Liu Z; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China., Zang W; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China., He C; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China., Li Z; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China., Ren W; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China., Chen T; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China., Xu C; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China., Zhang Y; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China. Electronic address: zhangyujie@nimte.ac.cn., Wu A; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China. Electronic address: aiguo@nimte.ac.cn., Lin J; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China. Electronic address: linjie@nimte.ac.cn.
Jazyk:	angličtina
Zdroj:	Biosensors & bioelectronics [Biosens Bioelectron] 2025 Jan 15; Vol. 268, pp. 116897. Date of Electronic Publication: 2024 Oct 30.
DOI:	10.1016/j.bios.2024.116897
Abstrakt:	Detecting circulating tumor cells (CTCs) from blood has become a promising approach for cancer diagnosis. Surface-enhanced Raman Spectroscopy (SERS) has rapidly developed as a significant detection technology for CTCs, offering high sensitivity and selectivity. Encoded SERS bioprobes have gained attention due to their excellent specificity and ability to identify tumor cells using Raman signals. Machine learning has also made significant contributions to biomedical applications, especially in medical diagnosis. In this study, we developed a detection strategy combining encoded SERS bioprobes and machine learning models to identify CTCs. Dual-modal SERS bioprobes were designed and co-incubated with tumor cells by the "cocktail" method. An identification model for CTCs was constructed using principal component analysis (PCA) and the Random Forest classification algorithm. This innovative strategy endows SERS bioprobes with both effective magnetic separation and highly sensitive identification of CTCs, even at low concentrations of 2 cells/mL. It achieved a high detection rate of 98% for CTCs and effectively eliminated interference from peripheral WBCs. This simple and efficient strategy provides a new approach for CTCs detection and holds important significance for cancer diagnosis. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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