LncRNA HULIB promotes LPS induced inflammatory response in bovine mammary epithelial cells via PP2AB.

Autor: Wang J; College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China., Wang X; College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China., Feng F; College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China., Pan C; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China., Lan X; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China., Luoreng Z; College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China. Electronic address: luorenzhuoma@nxu.edu.cn.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2024 Dec 25; Vol. 143 (Pt 3), pp. 113496. Date of Electronic Publication: 2024 Nov 01.
DOI: 10.1016/j.intimp.2024.113496
Abstrakt: Bovine mastitis is regulated by genetic and environmental factors. Long non-coding RNAs (LncRNAs), which regulate various biological processes (immune system and biological development), have been found to play a role in bovine mammary inflammation responses. Here, a novel functional lncRNA, named lncRNA HULIB, was identified as a regulator during bovine mastitis. qRT-PCR and subcellular fractionation assays showed that lncRNA HULIB was significantly up-regulated in LPS-induced bMECs and was mainly localized in the cytoplasm. Gain- or loss-of-function experiments demonstrated that an increase in lncRNA HULIB expression elevated the expression of TLR4 and NF-κB1, which enhanced NF-κB activity, promoting the expression of pro-inflammatory cytokines (IL-6, IL-8, IL-1β, etc) and apoptosis-related genes (BAX, CASP9 and CASP3, etc), while the expression of proliferation-related genes (PCNA, Cyclin D1, Cyclin D2, CDK4 and CDK2) was down-regulated. Ultimately, these changes exacerbated the LPS-induced inflammatory response. Mechanistically, RNA pull-down and RNA immunoprecipitation (RIP) assays revealed that lncRNA HULIB could directly bind the PP2AB protein to regulate inflammatory responses. Overall, lncRNA HULIB is a pro-inflammatory regulator, and its silencing can alleviate the inflammatory responses of bMECs, providing a potential strategy for molecular therapy of bovine mastitis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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