Profile of molecular markers of Sulfadoxine-Pyrimethamine-resistant Plasmodium falciparum in individuals living in southern area of Brazzaville, Republic of Congo.

Autor:	Djontu JC; Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Biotechnology Center, University of Yaounde I, Yaounde, Cameroon. Electronic address: cdjontu@yahoo.fr., Baina MT; Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo. Electronic address: bainamarcel@gmail.com., Mbama Ntabi JD; Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo. Electronic address: dollonmbama@gmail.com., Lissom A; Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Department of Zoology, Faculty of Science, University of Bamenda, Bamenda, Cameroon. Electronic address: lissomabel@yahoo.fr., Umuhoza DM; Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo. Electronic address: dieumerciumuhoza1@gmail.com., Assioro Doulamo NV; Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo. Electronic address: assiorodoulamonauraveil@gmail.com., Vouvoungui CJ; Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo. Electronic address: vjchristevy@gmail.com., Boumpoutou RK; Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo. Electronic address: reauchelvykamal@gmail.com., Mouanga AM; Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Faculté des Sciences de la Santé, Université Marien Ngouabi, Brazzaville, Congo. Electronic address: amouanga@fcrm-congo.com., Nguimbi E; Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo. Electronic address: etienne.nguimbi@umng.cg., Ntoumi F; Fondation Congolaise pour la Recherche Médicale, Brazzaville, Congo; Institute for Tropical Medicine, University of Tübingen, Tübingen, Germany. Electronic address: ffntoumi@hotmail.com.
Jazyk:	angličtina
Zdroj:	International journal for parasitology. Drugs and drug resistance [Int J Parasitol Drugs Drug Resist] 2024 Dec; Vol. 26, pp. 100569. Date of Electronic Publication: 2024 Oct 26.
DOI:	10.1016/j.ijpddr.2024.100569
Abstrakt:	Background: Although the seasonal and perennial malaria chemopreventions are not implemented in the Republic of Congo, resistance to Sulfadoxine-pyrimethamine (SP) threatens the intermittent preventive treatment during pregnancy (IPTp-SP) and others treatments using the drug. The objective of this study was to determine the prevalence of molecular markers of P.falciparum resistance to SP in individuals with microscopic malaria infection in the south of Brazzaville. Methods: Two parallel surveys (health facilities and community-based cross sectional studies) were carried out in urban and rural areas in southern Brazzaville. Between March and October 2021, blood samples were collected from 328 P. falciparum microscopic positive individuals (1-83 years old, and sex ratio female/male of 1.1) to characterize dhfr and dhps genes involved in the P.falciparum resistance to SP. Restriction Fragment Length Polymorphism PCR was used for the detection of mutations within these parasite genes. Results: High prevalence of mutations was reported within Pfdhfr gene: N51I; 328/328 (100%) ratio (prevalence) [95 CI uncertainty], C59R; 317/328 (96.6 %) [94.1-98.1%], S108N; 326/326 (100%), N164L; 3/326 (0.9%) [0.3-2.7%], and Pfdhps gene: A437G; 292/327 (89.3%) [85.5-92.2%], K540E; 140/327(42.8 %) [37.6-48.2%], A581G; 136/325 (41.8%) [36.6-42.3%]. The quintuple mutant (N51I + C59R + S108N + A437G + K540E) and sextuple mutant haplotypes (N51I + C59R + S108N + A437G + K540E + A581G) were reported for 11/144 (7.6%) [4.3-13.2%] and 5/144 (3.4%) [1.5-7.9%]) of the participants respectively. The K540E and A437G mutants were more prevalent in the rural community; 81/139 (58.3%) [50.0-66.1%] and 135/139 (97.1%) [92.8-98.9%] respectively) than in the urban community; 21/50 (46.3%) [33.7-59.4%] and 47/54(87.0%) [75.6-93.6%] (p = 0.004 and p˂0.0001 respectively) CONCLUSION: These results indicate high prevalence of SP resistance mutations within the dhfr and dhps genes of P. falciparum isolates circulating in study sites, which may limit the efficacy of treatments using SP in these settings. Competing Interests: Declaration of competing interest We declare that this manuscript is original, has not been published before and is not being considered for publication elsewhere. The authors declare that they have no competing interest, and there has been no significant financial support for this work that could have influenced its outcomes. (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
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