Host dependent specialized metabolism of nitrogen export in actinorhizal nodules induced by Frankia cluster-2.

Autor:	Berckx F; Department of Ecology, Environment and Plant Science, Stockholm University, 106 91 Stockholm, Sweden.; Department of Crop Production Ecology, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden., Van Nguyen T; Department of Ecology, Environment and Plant Science, Stockholm University, 106 91 Stockholm, Sweden., Hilker R; German Center for Infection Research, Institute for Medical Microbiology, Justus Liebig University Giessen, 35392 Giessen, Germany., Wibberg D; Center for Biotechnology (CeBiTec), Bielefeld University, D-33615 Bielefeld, Germany.; Institute of Bio- and Geosciences IBG-5, Computational Metagenomics, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany., Battenberg K; Department of Plant Sciences, University of California Davis, Davis CA 95616, USA.; Stillinger Herbarium, University of Idaho, Moscow ID 83844-3026, USA., Kalinowski J; Center for Biotechnology (CeBiTec), Bielefeld University, D-33615 Bielefeld, Germany., Berry A; Department of Plant Sciences, University of California Davis, Davis CA 95616, USA., Pawlowski K; Department of Ecology, Environment and Plant Science, Stockholm University, 106 91 Stockholm, Sweden.
Jazyk:	angličtina
Zdroj:	Journal of experimental botany [J Exp Bot] 2024 Nov 02. Date of Electronic Publication: 2024 Nov 02.
DOI:	10.1093/jxb/erae446
Abstrakt:	Frankia cluster-2 strains are diazotrophs that engage in root nodule symbiosis with actinorhizal plants of the Cucurbitales and the Rosales. Previous studies have shown that an assimilated nitrogen source, presumably arginine, is exported to the host in nodules of Datisca glomerata (Cucurbitales), while a different metabolite is exported in the nodules of Ceanothus thyrsiflorus (Rosales). To investigate if an assimilated nitrogen form is commonly exported to the host by cluster-2 strains, and which metabolite would be exported in Ceanothus, we analysed gene expression levels, metabolite profiles, and enzyme activities in nodules. We conclude that the export of assimilated nitrogen in symbiosis seems to be a common feature for Frankia cluster-2 strains, but which source is host-dependent. The export of assimilated ammonium to the host suggests that 2-oxoglutarate is drawn from the TCA cycle at a high rate. This specialised metabolism obviates the need for the reductive branch of the TCA cycle. We found several genes encoding enzymes of the central carbon and nitrogen metabolism were lacking in Frankia cluster-2 genomes: the glyoxylate shunt and succinate semialdehyde dehydrogenase. This led to a linearization of the TCA cycle, and we hypothesize this could explain the low saprotrophic potential of Frankia cluster-2. (© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
Databáze:	MEDLINE
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